Difference between revisions of "Kruse 2019 MiP2019"

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The preliminary tests show that recorded respirations are similar to those documented in humans. However, a lack of reproducibility of respirometric analysis with PBMC taken from the same horses was observed. Therefore, a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is, up to now, not possible to be established. Nevertheless, an efficient PBMC isolation protocol was established and mitochondrial respiration with respirometry was obtained. The cause of the variations within the same individual is still under examination.
 
The preliminary tests show that recorded respirations are similar to those documented in humans. However, a lack of reproducibility of respirometric analysis with PBMC taken from the same horses was observed. Therefore, a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is, up to now, not possible to be established. Nevertheless, an efficient PBMC isolation protocol was established and mitochondrial respiration with respirometry was obtained. The cause of the variations within the same individual is still under examination.
 
|editor=[[Plangger M]], [[Tindle-Solomon L]]
 
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|mipnetlab=BE Liege Votion DM
 
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|organism=Horse
 
|organism=Horse
 
|tissues=Blood cells
 
|tissues=Blood cells
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|instruments=Oxygraph-2k
 
|additional=PBMCs,
 
|additional=PBMCs,
 
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Latest revision as of 14:59, 12 September 2019

Caroline Kruse
Peripheral blood mononuclear cells to detect mitochondrial dysfunction in horses: a preliminary study.

Link: MiP2019

Kruse CJ, Art T, Mouithys-Mickalad A, Votion DM (2019)

Event: MiP2019

COST Action MitoEAGLE

Mitochondrial dysfunction has emerged as a ubiquitous cause of disease in human and animals. In the equine species, muscle fibers were analyzed to detect and assess defective mitochondrial function. Using respirometry, a so-called “mitochondrial syndrome” has been reported in equine atypical myopathy, which is characterized by a severe decrease of mitochondrial respiratory capacity in affected individuals [1].

Up to now, respirometric studies in horses have been successfully conducted on skeletal muscles samples. However, the muscle biopsy procedure is rather invasive and therefore difficult to use in equine clinical routine. Despite the fact that peripheral blood mononuclear cells (PBMC) have been used in human medicine to assess mitochondrial function, there is only one study comparing PBMC and skeletal muscle oxidative capacity in monkeys so far [2]. Therefore, the aim of this study was to test whether mitochondrial function can be assessed on equine blood cells with respirometry, according to the fact that blood is a biological sample easier to collect than muscle.

Whole blood was collected in 9 ml EDTA tubes in four horses. Cells of interest (PBMC) were isolated with a density-separation medium (Lymphoprep™) within one hour of sampling, following a standardized procedure. Electron paramagnetic resonance as well as the respirometric analysis were performed within the following 4 hours.

The preliminary tests show that recorded respirations are similar to those documented in humans. However, a lack of reproducibility of respirometric analysis with PBMC taken from the same horses was observed. Therefore, a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is, up to now, not possible to be established. Nevertheless, an efficient PBMC isolation protocol was established and mitochondrial respiration with respirometry was obtained. The cause of the variations within the same individual is still under examination.


Bioblast editor: Plangger M, Tindle-Solomon L O2k-Network Lab: BE Liege Votion DM


Labels: MiParea: Respiration  Pathology: Myopathy 

Organism: Horse  Tissue;cell: Blood cells 



HRR: Oxygraph-2k 

PBMCs 

Affiliations

Kruse CJ(1), Art T(1), Mouithys-Mickalad A(2), Votion DM(3)
  1. Dept functional sciences, Fac veterinary medicine
  2. Center Oxygen Research Development (CORD), Fac sciences
  3. Fundamental Applied Research Animals Health (FARAH), Fac veterinary medicine; Univ Liège (ULiège), Belgium

References

  1. Lemieux H, Boemer F, van Galen G, Serteyn, D, Amory H, Baise E, Cassart D, van Loon G, Marcillaud-Pitel C, Votion DM (2016) Mitochondrial function is altered in horse atypical myopathy. Mitochondrion 30:35-41.
  2. Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJA (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biology 10:65–77.