Difference between revisions of "Kudin 1999 Brain Res Brain Res Protoc"
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{{Publication | {{Publication | ||
|title=Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Quantitative investigation of mitochondrial function in single rat hippocampal slices: a novel application of high-resolution respirometry and laser-excited fluorescence spectroscopy. Brain Res. Protoc. 4: 329-334. | |title=Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Quantitative investigation of mitochondrial function in single rat hippocampal slices: a novel application of high-resolution respirometry and laser-excited fluorescence spectroscopy. Brain Res. Protoc. 4: 329-334. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/10592342 PMID: 10592342] | |||
|authors=Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS | |authors=Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS | ||
|year=1999 | |year=1999 | ||
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|abstract=Highly sensitive techniques are needed for the quantitative determination of mitochondrial oxidative phosphorylation function in single rat hippocampal slices or isolated hippocampal subfields. We determined the oxygen consumption of single hippocampal slices or subfields applying high-resolution respirometry adapted for slice measurements and measured the redox state of mitochondrial NAD(P)H in single hippocampal slices by laser-excited fluorimetry. These methods allow the sensitive detection of two parameters of mitochondrial oxidative phosphorylation which depend on supply of substrates and respiratory chain function. | |abstract=Highly sensitive techniques are needed for the quantitative determination of mitochondrial oxidative phosphorylation function in single rat hippocampal slices or isolated hippocampal subfields. We determined the oxygen consumption of single hippocampal slices or subfields applying high-resolution respirometry adapted for slice measurements and measured the redox state of mitochondrial NAD(P)H in single hippocampal slices by laser-excited fluorimetry. These methods allow the sensitive detection of two parameters of mitochondrial oxidative phosphorylation which depend on supply of substrates and respiratory chain function. | ||
|keywords=Hippocampal slice, Hippocampal subfield, Mitochondrial oxidative phosphorylation | |keywords=Hippocampal slice, Hippocampal subfield, Mitochondrial oxidative phosphorylation | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |||
|organism=Rat | |organism=Rat | ||
|tissues=Neurons; Brain | |tissues=Neurons; Brain | ||
|preparations=Permeabilized Cell or Tissue; Homogenate | |||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
}} | }} | ||
Revision as of 11:28, 6 September 2011
| Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Quantitative investigation of mitochondrial function in single rat hippocampal slices: a novel application of high-resolution respirometry and laser-excited fluorescence spectroscopy. Brain Res. Protoc. 4: 329-334. |
Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Brain Res. Protoc.
Abstract: Highly sensitive techniques are needed for the quantitative determination of mitochondrial oxidative phosphorylation function in single rat hippocampal slices or isolated hippocampal subfields. We determined the oxygen consumption of single hippocampal slices or subfields applying high-resolution respirometry adapted for slice measurements and measured the redox state of mitochondrial NAD(P)H in single hippocampal slices by laser-excited fluorimetry. These methods allow the sensitive detection of two parameters of mitochondrial oxidative phosphorylation which depend on supply of substrates and respiratory chain function. • Keywords: Hippocampal slice, Hippocampal subfield, Mitochondrial oxidative phosphorylation
Labels:
Organism: Rat
Tissue;cell: Neurons; Brain"Neurons; Brain" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
