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Difference between revisions of "Kuznetsov 1998 BTK"

From Bioblast
Line 14: Line 14:
|tissues=Heart, Skeletal muscle
|tissues=Heart, Skeletal muscle
|preparations=Permeabilized tissue, Isolated mitochondria
|preparations=Permeabilized tissue, Isolated mitochondria
|topics=ADP, O2
|topics=ADP, Oxygen kinetics
|couplingstates=LEAK, OXPHOS
|couplingstates=LEAK, OXPHOS
|substratestates=CI, CII
|substratestates=CI, CII

Revision as of 13:47, 1 December 2015

Publications in the MiPMap
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6.

» pdf: BTK1998: 273-276

Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Chalmers Reproservice

Abstract: Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca2+, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.


O2k-Network Lab: AT Innsbruck Gnaiger E


Labels: MiParea: Respiration, Instruments;methods 


Organism: Rat  Tissue;cell: Heart, Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria 

Regulation: ADP, Oxygen kinetics  Coupling state: LEAK, OXPHOS 

HRR: Oxygraph-2k