Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Kuznetsov 1998 BTK"

From Bioblast
 
(6 intermediate revisions by 5 users not shown)
Line 1: Line 1:
{{Publication
{{Publication
|title=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg: 273-6.
|title=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6.
|info=[http://www.oroboros.at/fileadmin/user_upload/1998_Kuznetsov-Gnaiger_BTK.pdf pdf: BTK1998: 273-276]
|info=[http://www.oroboros.at/fileadmin/user_upload/1998_Kuznetsov-Gnaiger_BTK.pdf pdf: BTK1998: 273-276]
|authors=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E
|authors=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger Erich
|year=1998
|year=1998
|journal=Chalmers Reproservice
|journal=Chalmers Reproservice
|abstract=Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca<sup>2+</sup>, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.
|abstract=Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca<sup>2+</sup>, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.
|mipnetlab=AT_Innsbruck_Gnaiger E
|mipnetlab=AT Innsbruck Gnaiger E
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}
Line 13: Line 13:
|organism=Rat
|organism=Rat
|tissues=Heart, Skeletal muscle
|tissues=Heart, Skeletal muscle
|preparations=Permeabilized tissue, Isolated Mitochondria
|preparations=Permeabilized tissue, Isolated mitochondria
|injuries=Hypoxia
|topics=ADP, Oxygen kinetics
|topics=ADP, O2
|couplingstates=LEAK, OXPHOS
|couplingstates=LEAK, OXPHOS
|substratestates=CI, CII
|pathways=N, S
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}

Latest revision as of 03:27, 23 November 2021

Publications in the MiPMap
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6.

» pdf: BTK1998: 273-276

Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger Erich (1998) Chalmers Reproservice

Abstract: Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca2+, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.


O2k-Network Lab: AT Innsbruck Gnaiger E


Labels: MiParea: Respiration, Instruments;methods 


Organism: Rat  Tissue;cell: Heart, Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria 

Regulation: ADP, Oxygen kinetics  Coupling state: LEAK, OXPHOS  Pathway: N, S  HRR: Oxygraph-2k