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Difference between revisions of "Kuznetsov 1998 BTK"

From Bioblast
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|tissues=Cardiac muscle, Skeletal muscle
|tissues=Cardiac muscle, Skeletal muscle
|preparations=Permeabilized tissue, Isolated Mitochondria
|preparations=Permeabilized tissue, Isolated Mitochondria
|couplingstates=OXPHOS
|kinetics=ADP; Pi, Oxygen
|kinetics=ADP; Pi, Oxygen
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}

Revision as of 14:21, 27 November 2012

Publications in the MiPMap
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg: 273-276.

» pdf: BTK1998: 273-276

Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Chalmers Reproservice

Abstract: Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca2+, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.


O2k-Network Lab: AT_Innsbruck_Gnaiger E


Labels:

Stress:Hypoxia  Organism: Rat  Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Skeletal muscle  Preparation: Permeabilized tissue, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 


Coupling state: OXPHOS 

HRR: Oxygraph-2k