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Kwast 1996 J Biol Chem

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Publications in the MiPMap
Kwast KE, Hand SC (1996) Acute depression of mitochondrial protein synthesis during anoxia. J Biol Chem 271:7313-9

Β» PMID: 8631750 Open Access

Kwast KE, Hand SC (1996) J Biol Chem

Abstract: Mitochondrial protein synthesis is acutely depressed during anoxia-induced quiescence in embryos of Artemia franciscana. Oxygen deprivation is accompanied in vivo by a dramatic drop in extramitochondrial pH, and both of these alterations strongly inhibit protein synthesis in isolated mitochondria. Here we show that the oxygen dependence is not explained simply by blockage of the Electron transfer-pathway or by the increased redox state. Whereas oxygen deprivation substantially depressed protein synthesis within 5 min and resulted in a 77% reduction after 1 h, aerobic incubations with saturating concentrations of cyanide or antimycin A had little effect during the first 20 min and only a modest effect after 1 h (36 and 20% reductions, respectively). Yet the mitochondrial NAD(P)H pools were fully reduced after 2–3 min with all three treatments. This cyanide- and antimycin-insensitive but hypoxia-sensitive pattern of protein synthesis depression suggests the presence of a molecular oxygen sensor within the mitochondrion. Second, we show for the first time that acidification of extramitochondrial pH exerts inhibition on protein synthesis specifically through changes in matrix pH. Matrix pH was 8.2 during protein synthesis assays performed at the extramitochondrial pH optimum of 7.5. When this proton gradient was abolished with nigericin, the extramitochondrial pH optimum for protein synthesis displayed an alkaline shift of 0.7 pH unit. These data suggest the presence of proton-sensitive translational components within the mitochondrion. β€’ Keywords: DatLab - separate application; Artemia franciscana

β€’ O2k-Network Lab: US LA Baton Rouge Hand SC, US IL Urbana Kwast K

Labels: MiParea: mt-Biogenesis;mt-density, Comparative MiP;environmental MiP, Developmental biology 

Stress:Ischemia-reperfusion  Organism: Artemia, Crustaceans 

Regulation: pH, Redox state