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Difference between revisions of "Larsen 2009 Diabetologia"

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{{Publication
{{Publication
|title=Larsen S, Ara I, Rabรธl R, Andersen JL, Boushel R, Dela F, Helge JW (2009) Are substrate use during exercise and mitochondrial respiratory capacity decreased in arm and leg muscle in type 2 diabetes? Diabetologia 52: 1400-1408.
|title=Larsen S, Ara I, Rabรธl R, Andersen JL, Boushel RC, Dela F, Helge JW (2009) Are substrate use during exercise and mitochondrial respiratory capacity decreased in arm and leg muscle in type 2 diabetes? Diabetologia 52:1400-8.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19396425 PMID: 19396425]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19396425 PMID: 19396425] ; [http://link.springer.com/article/10.1007%2Fs00125-009-1353-4 Open Access]
|authors=Larsen S, Ara I, Rabol R, Andersen JL, Boushel R, Dela F, Helge JW
|authors=Larsen S, Ara I, Raboel R, Andersen JL, Boushel RC, Dela F, Helge JW
|year=2009
|year=2009
|journal=Diabetologia
|journal=Diabetologia
|mipnetlab=DK_Copenhagen_BoushelR, DK Copenhagen DelaF,
|abstract=Aim/hypothesis: The aim of the study was to investigate mitochondrial function, fibre type distribution and substrate oxidation in arm and leg muscle during exercise in patients with type 2 diabetes and in obese and lean controls.Methods: Indirect calorimetry was used to calculate fat and carbohydrate oxidation during both progressive arm-cranking and leg-cycling exercises. Muscle biopsies from arm and leg were obtained. Fibre type, as well as O<sub>2</sub> flux capacity of saponin-permeabilised muscle fibres were measured, the latter by high resolution respirometry, in patients with type 2 diabetes, age- and BMI-matched obese controls, and age-matched lean controls.Results: Fat oxidation was similar in the groups during either arm or leg exercise. During leg exercise at higher intensities, but not during arm exercise, carbohydrate oxidation was lower in patients with type 2 diabetes compared with the other groups. In patients with type 2 diabetes, ADP-stimulated state 3 respiration per mg muscle with parallel electron input from complex I+II was lower in ''m. vastus lateralis'' compared with obese and lean controls, whereas no differences between groups were present in ''m. deltoideus''. A higher percentage of type IIX fibres was seen in ''m. vastus lateralis'' in patients with type 2 diabetes compared with obese and lean controls, whereas no difference was found in the deltoid muscle.Conclusions/interpretation: This study demonstrates similar O<sub>2</sub> flux capacity, fibre type distribution and carbohydrate oxidation in arm muscle in the groups despite the presence of attenuated values in leg muscle in patients with type 2 diabetes compared with obese and lean controls.
|abstract=Aim/hypothesis: The aim of the study was to investigate mitochondrial function, fibre type distribution and substrate oxidation in arm and leg muscle during exercise in patients with type 2 diabetes and in obese and lean controls.Methods: Indirect calorimetry was used to calculate fat and carbohydrate oxidation during both progressive arm-cranking and leg-cycling exercises. Muscle biopsies from arm and leg were obtained. Fibre type, as well as O<sub>2</sub> flux capacity of saponin-permeabilised muscle fibres were measured, the latter by high resolution respirometry, in patients with type 2 diabetes, age- and BMI-matched obese controls, and age-matched lean controls.Results: Fat oxidation was similar in the groups during either arm or leg exercise. During leg exercise at higher intensities, but not during arm exercise, carbohydrate oxidation was lower in patients with type 2 diabetes compared with the other groups. In patients with type 2 diabetes, ADP-stimulated state 3 respiration per mg muscle with parallel electron input from complex I+II was lower in ''m. vastus lateralis'' compared with obese and lean controls, whereas no differences between groups were present in ''m. deltoideus''. A higher percentage of type IIX fibres was seen in ''m. vastus lateralis'' in patients with type 2 diabetes compared with obese and lean controls, whereas no difference was found in the deltoid muscle.Conclusions/interpretation: This study demonstrates similar O<sub>2</sub> flux capacity, fibre type distribution and carbohydrate oxidation in arm muscle in the groups despite the presence of attenuated values in leg muscle in patients with type 2 diabetes compared with obese and lean controls.
|keywords=Exercise, Insulin, Lipid oxidation
|mipnetlab=SE Stockholm Boushel RC, DK Copenhagen Dela F, CA Vancouver Boushel RC, DK Copenhagen Larsen S
|discipline=Biomedicine
}}
}}
{{Labeling
{{Labeling
|discipline=Biomedicine
|injuries=RONS; Oxidative Stress, Mitochondrial Disease; Degenerative Disease and Defect
|organism=Human
|organism=Human
|tissues=Skeletal Muscle
|tissues=Skeletal muscle
|preparations=Permeabilized Cell or Tissue; Homogenate
|preparations=Permeabilized tissue
|enzymes=Complex I, Complex II; Succinate Dehydrogenase
|enzymes=Complex I, Complex II;succinate dehydrogenase
|kinetics=ADP; Pi, Oxygen, Reduced Substrate; Cytochrome c
|diseases=Diabetes
|topics=Respiration; OXPHOS; ETS Capacity, Redox State
|topics=ADP, Redox state, Substrate
|couplingstates=OXPHOS
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|discipline=Biomedicine
}}
}}

Latest revision as of 04:23, 8 February 2020

Publications in the MiPMap
Larsen S, Ara I, Rabรธl R, Andersen JL, Boushel RC, Dela F, Helge JW (2009) Are substrate use during exercise and mitochondrial respiratory capacity decreased in arm and leg muscle in type 2 diabetes? Diabetologia 52:1400-8.

ยป PMID: 19396425 ; Open Access

Larsen S, Ara I, Raboel R, Andersen JL, Boushel RC, Dela F, Helge JW (2009) Diabetologia

Abstract: Aim/hypothesis: The aim of the study was to investigate mitochondrial function, fibre type distribution and substrate oxidation in arm and leg muscle during exercise in patients with type 2 diabetes and in obese and lean controls.Methods: Indirect calorimetry was used to calculate fat and carbohydrate oxidation during both progressive arm-cranking and leg-cycling exercises. Muscle biopsies from arm and leg were obtained. Fibre type, as well as O2 flux capacity of saponin-permeabilised muscle fibres were measured, the latter by high resolution respirometry, in patients with type 2 diabetes, age- and BMI-matched obese controls, and age-matched lean controls.Results: Fat oxidation was similar in the groups during either arm or leg exercise. During leg exercise at higher intensities, but not during arm exercise, carbohydrate oxidation was lower in patients with type 2 diabetes compared with the other groups. In patients with type 2 diabetes, ADP-stimulated state 3 respiration per mg muscle with parallel electron input from complex I+II was lower in m. vastus lateralis compared with obese and lean controls, whereas no differences between groups were present in m. deltoideus. A higher percentage of type IIX fibres was seen in m. vastus lateralis in patients with type 2 diabetes compared with obese and lean controls, whereas no difference was found in the deltoid muscle.Conclusions/interpretation: This study demonstrates similar O2 flux capacity, fibre type distribution and carbohydrate oxidation in arm muscle in the groups despite the presence of attenuated values in leg muscle in patients with type 2 diabetes compared with obese and lean controls. โ€ข Keywords: Exercise, Insulin, Lipid oxidation

โ€ข O2k-Network Lab: SE Stockholm Boushel RC, DK Copenhagen Dela F, CA Vancouver Boushel RC, DK Copenhagen Larsen S


Labels: Pathology: Diabetes 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex II;succinate dehydrogenase  Regulation: ADP, Redox state, Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k