Difference between revisions of "Linearity"
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|description='''Linearity''' is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathematical transformation, to the concentration of the analyte in samples within a given range. This property is inherent in the [[Beer-Lambert law]] for [[absorbance]] alone, but deviations occur in [[scattering]] media. It is also a property of [[fluorescence]], but a [[fluorophore]] may not exhibit linearity, particularly over a large range of concentrations. | |description='''Linearity''' is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathematical transformation, to the concentration of the analyte in samples within a given range. This property is inherent in the [[Beer-Lambert law]] for [[absorbance]] alone, but deviations occur in [[scattering]] media. It is also a property of [[fluorescence]], but a [[fluorophore]] may not exhibit linearity, particularly over a large range of concentrations. | ||
|info=Paul WL (1991) USP perspectives on analytical methods evaluation. Pharm. Technol. 15: 130-141. Elwell CE (1995) A practical users guide to near infrared spectroscopy. Hamamatsu Photonics, 155pp. | |info=Paul WL (1991) USP perspectives on analytical methods evaluation. Pharm. Technol. 15: 130-141. Elwell CE (1995) A practical users guide to near infrared spectroscopy. Hamamatsu Photonics, 155pp. | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry, Spectrophotometry | |mitopedia method=Respirometry, Fluorometry, Spectrophotometry | ||
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{{MitoPedia O2k and high-resolution respirometry | |||
|mitopedia O2k and high-resolution respirometry=Oroboros QM | |||
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{{MitoPedia topics}} | {{MitoPedia topics}} | ||
Contributed by [[Harrison DK]], 2011-11-18 | |||
Latest revision as of 05:14, 19 July 2022
- high-resolution terminology - matching measurements at high-resolution
Linearity
Description
Linearity is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathematical transformation, to the concentration of the analyte in samples within a given range. This property is inherent in the Beer-Lambert law for absorbance alone, but deviations occur in scattering media. It is also a property of fluorescence, but a fluorophore may not exhibit linearity, particularly over a large range of concentrations.
Reference: Paul WL (1991) USP perspectives on analytical methods evaluation. Pharm. Technol. 15: 130-141. Elwell CE (1995) A practical users guide to near infrared spectroscopy. Hamamatsu Photonics, 155pp.
MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry
MitoPedia O2k and high-resolution respirometry:
Oroboros QM
Contributed by Harrison DK, 2011-11-18
