Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Ling 2019 Am J Clin Nutr"

From Bioblast
 
Line 1: Line 1:
{{Publication
{{Publication
|title=Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, NĂșñez Galindo A, CorthĂ©sy J, Dayon L, Charpagne A, MĂ©tairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, FĂ©asson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N (2019) Persistent low body weight in humans is associated with higher mitochondrial activity in white adipose tissue. Am J Clin Nutr [Epub ahead of print].
|title=Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, NĂșñez Galindo A, CorthĂ©sy J, Dayon L, Charpagne A, MĂ©tairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, FĂ©asson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N (2019) Persistent low body weight in humans is associated with higher mitochondrial activity in white adipose tissue. Am J Clin Nutr 110:605-16.
|info=[https://www.ncbi.nlm.nih.gov/pubmed/31374571 PMID: 31374571 Open Access]
|info=[https://www.ncbi.nlm.nih.gov/pubmed/31374571 PMID: 31374571 Open Access]
|authors=Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, Nunez Galindo A, Corthesy J, Dayon L, Charpagne A, Metairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, Feasson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N
|authors=Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, Nunez Galindo A, Corthesy J, Dayon L, Charpagne A, Metairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, Feasson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N

Latest revision as of 14:32, 15 October 2019

Publications in the MiPMap
Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, NĂșñez Galindo A, CorthĂ©sy J, Dayon L, Charpagne A, MĂ©tairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, FĂ©asson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N (2019) Persistent low body weight in humans is associated with higher mitochondrial activity in white adipose tissue. Am J Clin Nutr 110:605-16.

» PMID: 31374571 Open Access

Ling Y, Carayol J, Galusca B, Canto C, Montaurier C, Matone A, Vassallo I, Minehira K, Alexandre V, Cominetti O, Nunez Galindo A, Corthesy J, Dayon L, Charpagne A, Metairon S, Raymond F, Descombes P, Casteillo F, Peoc'h M, Palaghiu R, Feasson L, Boirie Y, Estour B, Hager J, Germain N, Gheldof N (2019) Am J Clin Nutr

Abstract: Constitutional thinness (CT) is a state of low but stable body weight (BMI ≀18 kg/m2). CT subjects have normal-range hormonal profiles and food intake but exhibit resistance to weight gain despite living in the modern world's obesogenic environment.

The goal of this study is to identify molecular mechanisms underlying this protective phenotype against weight gain.

We conducted a clinical overfeeding study on 30 CT subjects and 30 controls (BMI 20-25 kg/m2) matched for age and sex. We performed clinical and integrative molecular and transcriptomic analyses on white adipose and muscle tissues.

Our results demonstrate that adipocytes were markedly smaller in CT individuals (mean ± SEM: 2174 ± 142 ÎŒm2) compared with controls (3586 ± 216 ÎŒm2) (P < 0.01). The mitochondrial respiratory capacity was higher in CT adipose tissue, particularly at the level of complex II of the electron transport chain (2.2-fold increase; P < 0.01). This higher activity was paralleled by an increase in mitochondrial number (CT compared with control: 784 ± 27 compared with 675 ± 30 mitochondrial DNA molecules per cell; P < 0.05). No evidence for uncoupled respiration or "browning" of the white adipose tissue was found. In accordance with the mitochondrial differences, CT subjects had a distinct adipose transcriptomic profile [62 differentially expressed genes (false discovery rate of 0.1 and log fold change >0.75)], with many differentially expressed genes associating with positive metabolic outcomes. Pathway analyses revealed an increase in fatty acid oxidation ( P = 3 × 10-04) but also triglyceride biosynthesis (P = 3.6 × 10-04). No differential response to the overfeeding was observed in the 2 groups.

The distinct molecular signature of the adipose tissue in CT individuals suggests the presence of augmented futile lipid cycling, rather than mitochondrial uncoupling, as a way to increase energy expenditure in CT individuals. We propose that increased mitochondrial function in adipose tissue is an important mediator in sustaining the low body weight in CT individuals. This knowledge could ultimately allow more targeted approaches for weight management treatment strategies. This trial was registered at clinicaltrials.gov as NCT02004821.

Copyright © American Society for Nutrition 2019. ‱ Keywords: Constitutional thinness, Futile lipid cycling, Mitochondria, Proteomics, Respiration, Transcriptome analysis, White adipose tissue ‱ Bioblast editor: Plangger M ‱ O2k-Network Lab: CH Lausanne Canto C


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle, Fat  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k 

Labels, 2019-08