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| The two chamber approach of the O2k has among other benefits also advantages for [[Troubleshooting]]. By switching components between sides it usually is possible to locate a problem specifically at one component. This component can then be serviced by the user or replaced by OROBOROS Instruments without the need to ship the entire oxygraph.<br>
| | #REDIRECT [[O2k-Open Support]] |
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| __TOC__
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| == Locating a Problem by Switching Components ==
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| It is always important to access the performance of the instrument in a well defined manner. Therefore, at each stage of this process a full [[Sensor test]] has to be done. In such a sensor test all hardware problems are visible. Biological experiments are not suitable for trouble shooting purposes. For troubleshooting it is very helpful to observe the [[Raw signal]] rather than a calibrated signal.
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| For the terms used to denote different components please see the Start manual [http://www.oroboros.at/index.php?id=o2k-start MiPNet12.06], especially the figure in chapter "3. Assembly of the Oxygraph-2k".
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| #Perform a [[Sensor test]]. | |
| #Switch the [[OroboPOS| OroboPOS sensors]] between right and left side. This is also a good opportunity to clean and apply [[Contact oil]] to the gold pin and thread connecting the [[POS connector]]s and proper the proper [[OroboPOS]] sensors. When disconnecting a sensor from the POS connector remember to observe the guidelines to prevent damage by ESD, see [http://www.oroboros.at/index.php?id=esd-protection0 MiPNet14.1].
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| #Repeat the full [[Sensor test]]. If the problem moved together with the sensor from one side to the other, the problem is located in the [[sensor (POS).
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| #If the problem remained on the same side, switch the [[POS connector]] together with the attached sensors between right and left side.
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| #Repeat the full [[Sensor test]]. If the problem moved together with the POS connector, it is located at the [[OroboPOS]] sensor.
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| #If the problem still stays on the same side check carefully the DatLab settings for this side. There may be wrong settings for [[Gain]], [[Polarization voltage]] or [[Scaling]]. Or there may be a wrong calibration for this channel (this is easily detected by observing the [[Raw signal]]). When these settings have been ruled out consider next a problem related to [[Chamber assembly]]. Check by disassembling and reassembling the chamber. If there is any doubt about the [[glass chamber]] or the stirring bar they can be switched to the other chamber to confirm /localize a problem. Finally, when a malfunctioning chamber has been ruled out, an electronic defect in the [[Main unit]] for this channel must be considered. This should be confirmed by removing components as described below.<br>
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| {| cellspacing="1" cellpadding="1" border="1" align="center" width="513" style=""
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| |+ '''Summary'''
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| |-
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| ! scope="col" | Problem moves with
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| ! scope="col" | Problem situated at
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| |-
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| | [[POS]]
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| | [[POS]]
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| |-
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| | [[POS connector]]
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| | [[POS connector]]
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| |-
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| | not at all
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| | [[Chamber assembly]] or [[Main unit]]
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| |}
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| <br>
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| == Locating a Problem by Removing Components ==
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| The approach described above reached its limitations when more then one component is damaged. In this case some information can be gained by sequentially removing components. This approach can also be used to confirm a suspected defect in the main unit. When removing components from the running instrument is is of special importance to observe the [[ESD]] guidelines, see [http://www.oroboros.at/index.php?id=esd-protection [MiPNet14.1]]
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| Start with a fully assembled chamber, filled with some medium, e.g. water.
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| #Record the [[Raw signal]] at open chamber (same conditions as at the beginning of a [[Sensor test]]. If the signal is off scale (9.99 V) try to decrease the gain (Gain = 2 for atmospheric saturation).
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| #Empty the chamber. Remove the [[POS connector]] plus the still attached [[Polarographic oxygen sensor]]. Leave the sensor attached to the [[POS connector]]. Set an event, describing what you did and record the signal for a short time.
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| #Remove the [[Polarographic oxygen sensor]] from the [[POS connector]]. Set an event, describing what you did and record the signal for a short time.
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| #Remove the [[POS connector]] from the [[Main unit]]. Set an event, describing what you did and record the signal for a short time.
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| <br>
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| {| cellspacing="1" cellpadding="1" border="1" style="width: 745px; height: 114px;"
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| |+ '''Tested states and expected behavior'''
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| |-
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| | '''State'''
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| | '''Description'''
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| | '''Expected behavior'''
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| | '''I'''
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| | fully assembled, sensor in chamber, air saturation, Gain =2
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| | stable signal, 3 to 5 V
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| |-
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| | '''II'''
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| | POS connector + sensor removed from chamber, sensor still connected to sensor connector
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| | initial decrease in voltage
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| |-
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| | '''III'''
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| | sensor removed from POS connector
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| | stable signal at 0 V
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| |-
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| | '''IV'''
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| | POS connector removed from main unit
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| | for oxygraphs series A to C: typically signal off scale (+/- 9.99 V), for oxygraphs series D and higher: typically 0 V; may vary, a gain independent stable signal of 0.4 V is a strong indication of an electronic problem in the main unit
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| |}
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| <br>
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| {| cellspacing="1" cellpadding="1" border="1" style="width: 749px; height: 201px;"
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| |-
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| ! scope="col" colspan="4" | State<br>expected behavior observed? yes/no <br>
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| ! scope="col" rowspan="2" | Interpretation / Comment <br> <br>
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| |-
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| ! scope="col" | I
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| ! scope="col" | II
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| ! scope="col" | III
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| ! scope="col" | IV
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| |-
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| | no
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| | yes
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| | yes
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| | yes
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| | may indicate a problem in the [[Chamber assembly]], or a defective sensor, continue testing
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| |-
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| | no
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| | no/yes
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| | yes
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| | yes
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| | probable defect in the [[Sensor]]. This indication is not so strong as a localization obtained via switching components. The [[POS connector]] (not the [[Sensor]]) may be defect, but this may be only visible when a signal is present.
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| |-
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| | no
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| | no
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| | no
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| | yes
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| | indicates a defect in the [[POS connector]]
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| |-
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| | no
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| | no
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| | no
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| | no
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| | indicates a problem with [[Chamber assembly]] or a defect in the [[Main unit]]; if the problem can not be solved by reassembling the chamber, contact OROBOROS Instruments.
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| |}
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| <br>
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| {{#set:Technical service=O2 sensor|Technical service=POS connector| Technical service=Main unit}}
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| {{Troubleshooting}}
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