Malate

high-resolution terminology - matching measurements at high-resolution

Malate

Description

Malate and glutamate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations as mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Malate dehydrogenase located in the mitochondrial matrix oxidizes malate to oxaloacetate.

Abbreviation: M

Reference: Gnaiger 2012 MitoPathways, MiPNet09.12

MitoPedia topics: Substrate and metabolite 

Application in HRR

M: Malate (L-Malic acid, C₄H₆0₅); Sigma M 1000, 100 g, store at R.T.; FW = 134.1

Preparation of 400 mM stock solution (dissolved in H₂O):

1) Weigh 268.2 mg of L-Malic acid

2) Add 3 ml H₂O

3) Neutralize with 10 N KOH (approx. 350 µl)

4) Adjust final volume to 5 ml (in 5 mL volumetric glass flask)

5) Divide into 0.5 ml portions

6) Store frozen at -20 °C

Comment: 800 mM stock until 2013-11-20

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 5 µl using a 25 µl Hamilton syringe
• Final conc. in 2 ml O2k-chamber: 1 mM