Malate

high-resolution terminology - matching measurements at high-resolution

Malate

Description

Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Glutamate and malate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations.

Abbreviation: M

Reference: MiPNet11.04; MiPNet09.12

MitoPedia topics: Substrate and metabolite 

Application in HRR

M: Malate (L-Malic acid, C₄H₆0₅); Sigma M 1000, 100 g, store at R.T.: FW = 134.1)

Preparation of 800 mM stock solution (dissolved in H₂O):

1) weigh 536.5 mg of L-Malic acid

2) add 3 ml H₂O

3) neutralize with 10 N KOH (approx. 750 µl)

4) adjust final volume to 5 ml (in 5 mL volumetric glass flask)

5) divide into 0.2 ml portions

6) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 5 µl using a 25 µl Hamilton syringe
• Final conc. in 2 ml O2k-chamber: 2 mM