Martinez 2013 Marine Biol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Martinez E, Menze MA, Torres JJ (2013) Mitochondrial energetics of benthic and pelagic Antarctic teleosts. Marine Biol 160:2813- | |title=Martinez E, Menze MA, Torres JJ (2013) Mitochondrial energetics of benthic and pelagic Antarctic teleosts. Marine Biol 160:2813-23. | ||
|info=[http://link.springer.com/article/10.1007/s00227-013-2273-x 2273] | |info=[http://link.springer.com/article/10.1007/s00227-013-2273-x 2273] | ||
|authors=Martinez E, Menze MA, Torres JJ | |authors=Martinez E, Menze MA, Torres JJ | ||
|year=2013 | |year=2013 | ||
|journal=Marine Biol | |journal=Marine Biol | ||
|abstract=Antarctic fauna are highly adapted to the frigid waters of the Southern Ocean. This study describes the in vitro temperature sensitivity of oxygen consumption rates measured in liver mitochondria from the pelagic notothenioid Pleuragramma antarcticum between 5 and 35 °C. Oxygen fluxes were measured after the addition of millimolar levels of pyruvate, malate, succinate and glutamate ( | |abstract=Antarctic fauna are highly adapted to the frigid waters of the Southern Ocean. This study describes the ''in vitro'' temperature sensitivity of oxygen consumption rates measured in liver mitochondria from the pelagic notothenioid ''Pleuragramma antarcticum'' between 5 and 35 °C. Oxygen fluxes were measured after the addition of millimolar levels of pyruvate, malate, succinate and glutamate (LEAK) and saturating levels of ADP [oxidative phosphorylation (OXPHOS)]. OXPHOS respiration significantly decreased above 18.7 °C. A comparison of the oxidative capacities among ''P. antarcticum'' and other notothenioids showed significant differences in OXPHOS respiration, where benthic species exhibited about 50% lower OXPHOS capacities than ''P. antarcticum''. In addition, OXPHOS capacities normalized per milligram of mitochondrial protein of ''P''. antarcticum'' were up to eight times higher than those reported in the literature for other notothenioids. The comparatively high respiration rates measured in this study may be explained by our approach, which engaged both Complexes I and II under conditions of oxidative phosphorylation. OXPHOS capacities of independently activated Complexes I and II were found to range from 42 to 100% of rates obtained when both complexes were activated simultaneously in the same species. The remarkable tolerance of ''P. antarcticum'' OXPHOS toward warmer temperatures was unexpected for an Antarctic stenotherm and may indicate that thermal sensitivity of their mitochondria is not the driving force behind their stenothermy. | ||
|mipnetlab=US IL Charleston Menze MA, US LA Baton Rouge Hand SC | |mipnetlab=US IL Charleston Menze MA, US LA Baton Rouge Hand SC, US KY Louisville Menze MA | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
| | |organism=Fishes | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Homogenate | |preparations=Homogenate | ||
|topics=Temperature | |topics=Temperature | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
| | |pathways=N, S, NS | ||
}} | }} | ||
Latest revision as of 14:28, 28 March 2018
| Martinez E, Menze MA, Torres JJ (2013) Mitochondrial energetics of benthic and pelagic Antarctic teleosts. Marine Biol 160:2813-23. |
» 2273
Martinez E, Menze MA, Torres JJ (2013) Marine Biol
Abstract: Antarctic fauna are highly adapted to the frigid waters of the Southern Ocean. This study describes the in vitro temperature sensitivity of oxygen consumption rates measured in liver mitochondria from the pelagic notothenioid Pleuragramma antarcticum between 5 and 35 °C. Oxygen fluxes were measured after the addition of millimolar levels of pyruvate, malate, succinate and glutamate (LEAK) and saturating levels of ADP [oxidative phosphorylation (OXPHOS)]. OXPHOS respiration significantly decreased above 18.7 °C. A comparison of the oxidative capacities among P. antarcticum and other notothenioids showed significant differences in OXPHOS respiration, where benthic species exhibited about 50% lower OXPHOS capacities than P. antarcticum. In addition, OXPHOS capacities normalized per milligram of mitochondrial protein of P. antarcticum were up to eight times higher than those reported in the literature for other notothenioids. The comparatively high respiration rates measured in this study may be explained by our approach, which engaged both Complexes I and II under conditions of oxidative phosphorylation. OXPHOS capacities of independently activated Complexes I and II were found to range from 42 to 100% of rates obtained when both complexes were activated simultaneously in the same species. The remarkable tolerance of P. antarcticum OXPHOS toward warmer temperatures was unexpected for an Antarctic stenotherm and may indicate that thermal sensitivity of their mitochondria is not the driving force behind their stenothermy.
• O2k-Network Lab: US IL Charleston Menze MA, US LA Baton Rouge Hand SC, US KY Louisville Menze MA
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Organism: Fishes
Tissue;cell: Liver
Preparation: Homogenate
Regulation: Temperature Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS
