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McBride 2019 Arch Biochem Biophys - Revision history
2024-03-29T11:38:23Z
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Gnaiger Erich at 11:22, 7 March 2020
2020-03-07T11:22:43Z
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Gnaiger Erich
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Krumschnabel Gerhard at 09:56, 23 May 2019
2019-05-23T09:56:31Z
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Krumschnabel Gerhard
https://wiki.oroboros.at/index.php?title=McBride_2019_Arch_Biochem_Biophys&diff=177608&oldid=prev
Plangger Mario at 12:05, 17 April 2019
2019-04-17T12:05:50Z
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3. Arch Biochem Biophys <del style="font-weight: bold; text-decoration: none;">[Epub ahead of print]</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3. Arch Biochem Biophys <ins style="font-weight: bold; text-decoration: none;">663:239-48</ins>.</div></td></tr>
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Plangger Mario
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Plangger Mario at 12:45, 23 January 2019
2019-01-23T12:45:53Z
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Plangger Mario
https://wiki.oroboros.at/index.php?title=McBride_2019_Arch_Biochem_Biophys&diff=171216&oldid=prev
Plangger Mario: Created page with "{{Publication |title=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncou..."
2019-01-23T12:45:32Z
<p>Created page with "{{Publication |title=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncou..."</p>
<p><b>New page</b></p><div>{{Publication<br />
|title=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3. Arch Biochem Biophys [Epub ahead of print].<br />
|info=[https://www.ncbi.nlm.nih.gov/pubmed/30659802 PMID: 30659802]<br />
|authors=McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME<br />
|year=2019<br />
|journal=Arch Biochem Biophys<br />
|abstract=Mitochondrial reactive oxygen species (ROS) are important cellular signaling molecules, but can cause oxidative damage if not kept within tolerable limits. An important proximal form of ROS in mitochondria is superoxide. Its production is thought to occur in regulated stochastic bursts, but current methods using mitochondrial targeted cpYFP to assess superoxide flashes are confounded by changes in pH. Accordingly, these flashes are generally referred to as 'mitoflashes'. Here we provide regulatory insights into mitoflashes and pH fluctuations in skeletal muscle, and the role of uncoupling protein-3 (UCP3). Using quantitative confocal microscopy of mitoflashes in intact muscle fibers, we show that the mitoflash magnitude significantly correlates with the degree of mitochondrial inner membrane depolarization and ablation of UCP3 did not affect this correlation. We assessed the effects of the absence of UCP3 on mitoflash activity in intact skeletal muscle fibers, and found no effects on mitoflash frequency, amplitude or duration, with a slight reduction in the average size of mitoflashes. We further investigated the regulation of pH flashes (pHlashes, presumably a component of mitoflash) by UCP3 using mitochondrial targeted SypHer (mt-SypHer) in skeletal muscle fibers. The frequency of pHlashes was significantly reduced in the absence of UCP3, without changes in other flash properties. ROS scavenger, tiron, did not alter pHlash frequency in either WT or UCP3KO mice. High resolution respirometry revealed that in the absence of UCP3 there is impaired proton leak and Complex I-driven respiration and maximal coupled respiration. Total cellular production of hydrogen peroxide (H2O2) as detected by Amplex-UltraRed was unaffected. Altogether, we demonstrate a correlation between mitochondrial membrane potential and mitoflash magnitude in skeletal muscle fibers that is independent of UCP3 and a role for UCP3 in the control of pHlash frequency and of proton leak- and Complex I coupled-respiration in skeletal muscle fibers. The differential regulation of mitoflashes and pHlashes by UCP3 and tiron also indicate that the two events, though may be related, are not identical events.<br />
<br />
<small>Copyright © 2019. Published by Elsevier Inc.</small><br />
|keywords=Electron transport chain, Mitochondrial flashes, Proton leak, Reactive oxygen species, Superoxide, Uncoupling protein 3, pH<br />
|editor=[[Plangger M]],<br />
|mipnetlab=CA Ottawa Harper ME<br />
}}<br />
{{Labeling<br />
|area=Respiration, Genetic knockout;overexpression<br />
|injuries=Oxidative stress;RONS<br />
|organism=Mouse<br />
|tissues=Skeletal muscle<br />
|preparations=Permeabilized tissue<br />
|enzymes=Uncoupling protein<br />
|couplingstates=LEAK, OXPHOS<br />
|pathways=F, N, CIV, NS, ROX<br />
|instruments=Oxygraph-2k, O2k-Fluorometer<br />
|additional=Labels, 2019-01, Amplex UltraRed<br />
}}</div>
Plangger Mario