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== MitoEAGLE Short-Term Scientific Mission ==
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_1 |STSM Grant Period 1]]
::: '''Motivation letter'''
::::My name is András Mészáros and I am working as associate professor in the Institute of Surgical Research at the University of Szeged, Hungary. I got my M.D. degree in 2012 and I was working as PhD fellow in the same institute for three years subsequently before taking the current position. My main research interest is to understand mitochondrial dysfunction during oxido-reductive stress conditions (hypoxia, ischemia-reperfusion) in animal models and cultured cells. My further aim is to characterize acting mechanism of novel biologically active gaseous compounds in mitochondria. I have been working with High Resolution Respirometry and basic fluorometry for three years, gaining experience maínly with tissue homogenate and isolated mitochondría. An essential step now is to extend my expertise toward assessment of other functions than oxygen consumption.
::::The main objective of the planned STSM training is to get acquainted with and to standardize fluorometric measurements of isolated mitochondria and cultured cells. During the 8 days of short-term visit at the MitoFit Laboratory three new techniques could be learned:(l) measurement of reactive oxygen species production of permeabilized cells using H2O2-sensitive fluorescent probes; (2) determination of mitochondrial membrane potential and (3) assessment of mitochondrial phosphate/oxygen ratio by measuring ATP levels fluorometrically. Preparation and execution of each method will be learned with emphasis on internal quality control and possible adaptation of measurement protocols to samples from various tissues. Furthermore, we are going to use tools of complex data analysis and calibration.
::::Fulfillment of the proposed research plan during the STSM training would contribute significantly to the objectives of the MitoEAGLE Working Group 1 (standardization of protocols in respirometry).I wish to actively participate in the MitoEAGLE project, in, among others, establishing and testing reference protocols to provide basis for comparison of results obtained in various laboratories. To this end, a short stay at the MitoFit Laboratory of Oroboros Instruments could provide a perfect platform having up-to-date instrumental background, well established measurement protocols and top level proficiency.
::: '''STSM request''': [http://www.mitoglobal.org/images/7/7f/Meszaros_A_STSM_Request_CA_15203.pdf ''download pdf'']
== MitoEAGLE ==
****: '''Benefits for COST and for the COST Action MitoEAGLE'''
:::: I am working mainly with immune cells from blood and liver mitochondria, while enterocytes and epithelial cells are the major targets of my plans for the next few months.
== Participated at ==
::::* [[Bioblast 2022|Bioblast 2022: BEC Inaugural Conference]]
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]
::::* [[DSL Retreat 2019 Innsbruck AT]]
::::* [[TRANSMIT Kick-off meeting Bologna IT| TRANSMIT Kick-off meeting 2018 Bologna IT]]
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]
::::* [[EBEC2018 Budapest HU]]
::::* [[IOC132|IOC132 Budapest HU]]
::::* [[Mitochondrial Medicine 2018 Hinxton UK]]
::::* [[MiPNet23.03 IOC128 Cambridge UK| IOC128 Cambridge UK]]
::::* [[IOC127|IOC127 Szeged HU]]
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]
::::* [[IOC124|IOC124 Schroecken AT]]
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]
::::* [[MitoFit_Open_Seminar_2017-07-14| MitoFit Open Seminar 2017 Innsbruck AT]]
::::* [[IOC116 | IOC116 Innsbruck AT]]
::::* [[MiP2014 | MiP2014 Obergurgl AT]]
::::* [[MiPNet18.09_IOC80|IOC80 Schroecken AT]]
::::* [[IOC79|IOC79 Obergurgl AT]]
== Visiting scientist in the Oroboros O2k-Laboratory ==
[[Meszaros Andras| András Mészáros ]]: Visiting scientist at the [[Oroboros Laboratories: visiting scientists |Oroboros O2k-Laboratory]]
::::::* 2016, November 21 to 28
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