Difference between revisions of "MiPNet06.03 POS-calibration-SOP"
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'''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-calibration]]). This is part 1 of O2k Quality Control. | '''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-calibration]]). This is part 1 of O2k Quality Control. | ||
:» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 Instrumental O2 background]] | :» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 Instrumental O2 background]] | ||
:» O2k-Manual | :» O2k-Manual: [[MiPNet19.18D_O2k-calibration]] | ||
:» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | :» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | ||
:» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]] | :» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]] | ||
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== Further details == | == Further details == | ||
[[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: | [[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: [[MiPNet14.06 Instrumental O2 background]] | ||
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== References == | == References == | ||
::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast | ::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast link]] | ||
::::* [[ | ::::* [[Gnaiger 2008 POS]] | ||
::::* [[MiPNet19. | ::::* [[MiPNet19.18D O2k-calibration]] | ||
::: '''This article is cited in''' | ::: '''This article is cited in''' | ||
::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 »PubMed | ::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 »PubMed 18370948] | ||
[[Category:Technical service]] | [[Category:Technical service]] |
Revision as of 15:57, 13 December 2016
MiPNet06.03 POS-calibration-SOP
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. |
OROBOROS (2016-02-09) Mitochondr Physiol Network
Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8. » Versions
O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-calibration). This is part 1 of O2k Quality Control.
- » O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 Instrumental O2 background
- » O2k-Manual: MiPNet19.18D_O2k-calibration
- » Product: OroboPOS, O2k, O2k-Catalogue
- » Template: O2-calibration.xlsx
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP
Further details
Next step - O2k Quality Control 2: MiPNet14.06 Instrumental O2 background
References
- Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - »Bioblast link
- Gnaiger 2008 POS
- MiPNet19.18D O2k-calibration
- This article is cited in
- Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948
- This article is cited in