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Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

From Bioblast
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'''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-calibration]]). This is part 1 of O2k Quality Control.
'''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-calibration]]). This is part 1 of O2k Quality Control.


:» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 Instrumental O2 background]]«
:» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 Instrumental O2 background]]
:» O2k-Manual »[[MiPNet19.18D_O2k-calibration]]«
:» O2k-Manual: [[MiPNet19.18D_O2k-calibration]]
:» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]]
:» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]]
:» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]]
:» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]]
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== Further details ==
== Further details ==


[[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: »[[MiPNet14.06 Instrumental O2 background]]«
[[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: [[MiPNet14.06 Instrumental O2 background]]




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== References ==
== References ==
::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast link«]]
::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast link]]
::::* [[Gnaiger_2008_POS]]
::::* [[Gnaiger 2008 POS]]
::::* [[MiPNet19.18D_O2k-calibration]]
::::* [[MiPNet19.18D O2k-calibration]]


::: '''This article is cited in'''
::: '''This article is cited in'''
::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 »PubMed 18370948«]
::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 »PubMed 18370948]




[[Category:Technical service]]
[[Category:Technical service]]

Revision as of 15:57, 13 December 2016

                



MiPNet06.03 POS-calibration-SOP

Publications in the MiPMap
O2k-Protocols
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

» Bioblast pdf

OROBOROS (2016-02-09) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8. » Versions

O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-calibration). This is part 1 of O2k Quality Control.

» O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 Instrumental O2 background
» O2k-Manual: MiPNet19.18D_O2k-calibration
» Product: OroboPOS, O2k, O2k-Catalogue
» Template: O2-calibration.xlsx


O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Protocol 

O2k-SOP 


Further details

O2k-QCS Next step - O2k Quality Control 2: MiPNet14.06 Instrumental O2 background


cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.

References

This article is cited in
  • Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948