MiPNet06.03 POS-calibration-SOP
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OROBOROS (2016-02-09) Mitochondr Physiol Network
Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8. » Versions
O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-calibration). This is part 1 of O2k Quality Control.
- » O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 Instrumental O2 background
- » O2k-Manual: MiPNet19.18D_O2k-calibration
- » Product: OroboPOS, O2k, O2k-Catalogue
- » Template: O2-calibration.xlsx
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP
Further details
Next step - O2k Quality Control 2: MiPNet14.06 Instrumental O2 background
References
- Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - »Bioblast link
- Gnaiger 2008 POS
- MiPNet19.18D O2k-calibration
- This article is cited in
- Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948
- This article is cited in
