Difference between revisions of "MiPNet06.06 Chemical O2 background"

Revision as of 12:51, 26 November 2019

Oxygraph assay of cytochrome c oxidase activity: chemical background correction.

» » Versions

Oroboros (2015-04-27) Mitochondr Physiol Network

Abstract: Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome c oxidase activity: chemical background correction. Mitochondr Physiol Network 06.06(07):1-4.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

» Product: O2k-Catalogue: O2k-MultiSensor, O2k-Core, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_Oroboros

@@ Line 5: / Line 5: @@
 |year=2015-04-27
 |journal=Mitochondr Physiol Network
 |abstract=Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome ''c'' oxidase activity: chemical background correction. Mitochondr Physiol Network 06.06(07):1-4.
-After sequential titration of ascorbate (added first) and TMPD (with or without added cytochrome c), the total oxygen flux increases due to (i) COX activity and (ii) autooxidation of ascrobate and TMPD. Correction for autooxidation is a routine procedure in high-resolution respirometry, but it requires precautions. We recommend, therefore, evaluation of chemical background effects by application of inhibitors of cytochrome c oxidase (cyanide, azide) subsequent to recording of flux with ascorbate and TMPD. After full inhibitioin of COX, a small non-COX component of biological oxidations and the large effect of autooxidation are recorded over a range of further declining oxygen concentration. After re-oxygenation, inhibited oxygen flux is recorded at oxygen levels above and within the oxygen concentration pertaining to the COX activity measurment. This provides a complete internal calibration of the chemical background, as a function of oxygen concentration.
 {{MiPNet pdf page linking to MitoPedia}}
 :» Product: O2k-Catalogue: [[O2k-MultiSensor]], [[O2k-Core]], [[O2k-Catalogue]]
 |mipnetlab=AT_Innsbruck_Oroboros
-}}
-{{Labeling
-|area=Instruments;methods
-|preparations=Enzyme, Oxidase;biochemical oxidation
-|enzymes=Complex IV;cytochrome c oxidase
-|instruments=Oxygraph-2k, O2k-Manual
-|additional=O2k-SOP
 }}