MiPNet08.12 IOC22: Difference between revisions
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. | ||
|info=[[Media:MiPNet08.12 NO-O2kWorkshop.pdf|'''Open Access-MiPNet08.12.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet08.12_NO-O2kWorkshop.pdf Versions] | |info=[[Media:MiPNet08.12 NO-O2kWorkshop.pdf|'''Open Access-MiPNet08.12.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet08.12_NO-O2kWorkshop.pdf Versions] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year= | |year=2011-12 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM ( | |abstract='''Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2011) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12(07).''' | ||
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa). | A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa). | ||
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See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics] | See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW}} | |mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW | ||
}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods | |area=Respiration, Instruments;methods | ||
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|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, NO, Protocol | |instruments=Oxygraph-2k, NO, Protocol | ||
|additional=O2k-Demo, O2k-MultiSensor}} | |additional=O2k-Demo, O2k-MultiSensor | ||
}} | |||
{{Mainpage OROBOROS}} | |||
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[[Category:OroboPedia]] | [[Category:OroboPedia]] |
Revision as of 19:26, 14 June 2014
NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. |
ยป Open Access-MiPNet08.12.pdf; Versions
OROBOROS (2011-12) Mitochondr Physiol Network
Abstract: Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2011) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12(07).
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Reference: Biochim Biophys Acta 1797: 557-565 (2010)
See also: Combined O2 and NO measurement and oxygen kinetics
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia, RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Liver Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor