Difference between revisions of "MiPNet08.16 pH calibration"
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|title=Gnaiger E. Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16. | |title=Gnaiger E. Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16. | ||
|info=[[Media:MiPNet08.16 pH-Calibration.pdf|'''Open Access-MiPNet08.16.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet08.16_pH-Calibration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | |info=[[Media:MiPNet08.16 pH-Calibration.pdf|'''Open Access-MiPNet08.16.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet08.16_pH-Calibration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|authors=OROBOROS, | |authors=OROBOROS, | ||
|year=2003 | |year=2003 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''O2k-Protocol for pH Measurement and Temperature Dependence of pH ''' | |abstract='''O2k-Protocol for pH Measurement and Temperature Dependence of pH ''' | ||
pH of blood and intracellular compartments is tightly regulated. Blood pH at 37 °C is 7.4. However, when temperature is lowered, the pH of blood and of intracellular buffer systems increases, which has been reported as early as 1927 but was ignored for the following 30 years. The phenomenon of a change in blood pH with temperature of about -0.016 U/°C was rediscovered by comparative physiologists in studies of "cold blooded" vertebrates (turtles, fish), and is was recognized soon that the same pH/temperature relation applies to "warm blooded" mammals (rat, human). The physicochemical basis of pH/temperature relations and the consequences for acid-base balance and protein function were primarily analyzed by Rahn and Reeves (1979). | |||
FIGURES HERE | |||
Besides the importance for physiological and biochemical systems, the temperature dependence of pH of buffer systems has experimental significance for the measurement of pH at different temperatures, and for the choice of buffer systems when designing experiments at various temperatures. | |||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|keywords=HRR-MultiSensor | |keywords=HRR-MultiSensor | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|topics=pH, Temperature | |topics=pH, Temperature | ||
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|additional=O2k-SOP | |additional=O2k-SOP | ||
}} | }} | ||
== Further information == | |||
See [http://wiki.oroboros.at/index.php/PH_electrode O2k-pH ISE-Module] for further information | |||
In [http://www.oroboros.at/index.php?id=datlab DatLab], the two-point calibration calculations are performed automatically (see equations in MiPNet12.08). | |||
:>> [http://www.oroboros.at/?protocols_ph_calib MiPNet08.16] | :>> [http://www.oroboros.at/?protocols_ph_calib MiPNet08.16] | ||
Revision as of 12:32, 29 November 2013
| Gnaiger E. Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16. |
» Open Access-MiPNet08.16.pdf; Versions
OROBOROS (2003) Mitochondr Physiol Network
Abstract: O2k-Protocol for pH Measurement and Temperature Dependence of pH
pH of blood and intracellular compartments is tightly regulated. Blood pH at 37 °C is 7.4. However, when temperature is lowered, the pH of blood and of intracellular buffer systems increases, which has been reported as early as 1927 but was ignored for the following 30 years. The phenomenon of a change in blood pH with temperature of about -0.016 U/°C was rediscovered by comparative physiologists in studies of "cold blooded" vertebrates (turtles, fish), and is was recognized soon that the same pH/temperature relation applies to "warm blooded" mammals (rat, human). The physicochemical basis of pH/temperature relations and the consequences for acid-base balance and protein function were primarily analyzed by Rahn and Reeves (1979).
FIGURES HERE
Besides the importance for physiological and biochemical systems, the temperature dependence of pH of buffer systems has experimental significance for the measurement of pH at different temperatures, and for the choice of buffer systems when designing experiments at various temperatures.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• Keywords: HRR-MultiSensor
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Regulation: pH, Temperature
HRR: pH, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP
Further information
See O2k-pH ISE-Module for further information
In DatLab, the two-point calibration calculations are performed automatically (see equations in MiPNet12.08).
- >> MiPNet08.16
