Difference between revisions of "MiPNet09.12 O2k-Titrations"
Line 8: | Line 8: | ||
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations. | A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations. | ||
:» | :» [[O2k-Protocols]] | ||
:» Product: [[Oxygraph-2k |OROBOROS O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | :» Product: [[Oxygraph-2k |OROBOROS O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS |
Revision as of 10:11, 31 July 2015
Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. »Bioblast pdf« |
» Versions
OROBOROS (2015-02-12) Mitochondr Physiol Network
Abstract: OROBOROS (2014) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(11):1.
A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.
- » O2k-Protocols
- » Product: OROBOROS O2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Inhibitor, Substrate, Uncoupler, Amino acid
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-chemicals and media
Further information
- » Excel Templates and DatLab-Demo Files
- » MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.