MiPNet12.17 BovineHeartMito

Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.

»  »Versions

Oroboros (2011-12-11) Mitochondr Physiol Network

Abstract: Petrosyan S, Naviaux R, Haas RH, Gnaiger E (2011) Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17(04).

The following demo experiment was performed in preparation for the lecture hall experiment, presented at the Mitochondrial Medicine conference (Diagnosis of Disease: Workshop on HRR at Mitochondrial Medicine 2007, UMDF, San Diego, USA, June 2007 (IOC40). For explanation of symbols, see Gnaiger 2012 MitoPathways.

Figure 1. Simultaneous display of oxygen concentration (blue lines) and oxygen flux (respiratory rate, red lines; negative time derivative of oxygen concentration) in chamber A and B.

>> Product: Oroboros O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Respiration, Instruments;methods 

Organism: Bovines  Tissue;cell: Heart  Preparation: SMP 

Regulation: Coupling efficiency;uncoupling, Cyt c  Coupling state: OXPHOS  Pathway: S  HRR: Oxygraph-2k, O2k-Protocol 

O2k-Demo, O2k-Core 

Further information

>> Excel Templates and DatLab-Demo Files