Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet17.02 PBI-Shredder manual"

From Bioblast
Line 3: Line 3:
{{Publication
{{Publication
|title=[[Image:O2k-Manual.jpg|right|70px|link=http://wiki.oroboros.at/index.php/O2k-Manual|O2k-Manual]] PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.  
|title=[[Image:O2k-Manual.jpg|right|70px|link=http://wiki.oroboros.at/index.php/O2k-Manual|O2k-Manual]] PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.  
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c7/MiPNet17.02_PBI-Shredder_HRR-Set.pdf |Bioblast pdf]]  
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c7/MiPNet17.02_PBI-Shredder_HRR-Set.pdf |Bioblast pdf]]  » [http://www.bioblast.at/index.php/File:MiPNet17.02_PBI-Shredder_HRR-Set.pdf Versions]
|authors=Oroboros
|authors=Oroboros
|year=2013-11-22
|year=2013-11-22
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:PBI-Shredder HRR-Set.JPG|180px|right|link=http://www.bioblast.at/index.php/PBI-Shredder_HRR-Set]]
|abstract=[[Image:PBI-Shredder HRR-Set.JPG|180px|right|link=http://www.bioblast.at/index.php/PBI-Shredder_HRR-Set]]
'''Draxl A, Eigentler A, Gnaiger E (2013) PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02(03):1-9.''' » [http://www.bioblast.at/index.php/File:MiPNet17.02_PBI-Shredder_HRR-Set.pdf Versions]
'''Draxl A, Eigentler A, Gnaiger E (2013) PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02(03):1-9.'''


The PBI-Shredder HRR-Set is an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues with high reproducibility of mitochondrial yield and mitochondrial function. In this manual for applications with high-resolution respirometry (HRR), we refer to the PBI User Manual with safety information, product use limitations and warranty information, and the Product Specification Sheet by Pressure BioSciences Inc. (PBI).
The PBI-Shredder HRR-Set is an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues with high reproducibility of mitochondrial yield and mitochondrial function. In this manual for applications with high-resolution respirometry (HRR), we refer to the PBI User Manual with safety information, product use limitations and warranty information, and the Product Specification Sheet by Pressure BioSciences Inc. (PBI).

Revision as of 09:14, 11 January 2018

                



MiPNet17.02 PBI-Shredder manual


Publications in the MiPMap
O2k-Manual
PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.

» Bioblast pdf  » Versions

Oroboros (2013-11-22) Mitochondr Physiol Network

Abstract:

PBI-Shredder HRR-Set.JPG

Draxl A, Eigentler A, Gnaiger E (2013) PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02(03):1-9.

The PBI-Shredder HRR-Set is an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues with high reproducibility of mitochondrial yield and mitochondrial function. In this manual for applications with high-resolution respirometry (HRR), we refer to the PBI User Manual with safety information, product use limitations and warranty information, and the Product Specification Sheet by Pressure BioSciences Inc. (PBI).

» Product: Oroboros O2k, Oroboros O2k-Catalogue


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom


Labels: MiParea: Instruments;methods 



Preparation: Homogenate 



HRR: O2k-Manual, O2k-Protocol 

Auxiliary, Mt-Preparation, PBI-Shredder 

Further information

» PBI-Shredder HRR-Set

List of publications: PBI-Shredder

 YearReferenceMammal and modelTissue and cellStressDiseases
Latina 2021 Acta Neuropathol Commun2021Latina V, Giacovazzo G, Cordella F, Balzamino BO, Micera A, Varano M, Marchetti C, Malerba F, Florio R, Ercole BB, La Regina F, Atlante A, Coccurello R, Di Angelantonio S, Calissano P, Amadoro G (2021) Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease. Acta Neuropathol Commun 9:38.MouseNervous systemAlzheimer's
MiPNet18.05 Amplex-Mouse-heart2019-06-24
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
MouseHeart
Bajzikova 2019 Cell Metab2019Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal GJ, Magalhaes Novais S, Olsinova M, Krobova L, An YJ, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim TL, Hozak P, Sardao VA, Koopman Werner JH, Ricchetti M, Oliveira PJ, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J (2019) Reactivation of dihydroorotate dehydrogenase-driven pyrimidine biosynthesis restores tumor growth of respiration-deficient cancer cells. Cell Metab 29:399-416.MouseEndothelial;epithelial;mesothelial cell
Genital
Other cell lines
Cancer
Haegler 2017 PLOS ONE2017Haegler P, Grünig D, Berger B, Terracciano L, Krähenbühl S, Bouitbir J (2017) Hepatic effects of pharmacological doses of hydroxy-cobalamin[c-lactam] in mice. PLOS ONE 12:e0171026.MouseLiver
Khalifa 2017 Physiol Rep2017Khalifa AR, Abdel-Rahman EA, Mahmoud AM, Ali MH, Noureldin M, Saber SH, Mohsen M, Ali SS (2017) Sex-specific differences in mitochondria biogenesis, morphology, respiratory function, and ROS homeostasis in young mouse heart and brain. Physiol Rep 5. pii: e13125.MouseHeart
Nervous system
Aging;senescence
Cardiovascular
Other
Torres 2016 Redox Biol2016Torres S, Matías N, Baulies A, Nuñez S, Alarcon-Vila C, Martinez L, Nuño N, Fernandez A, Caballeria J, Levade T, Gonzalez-Franquesa A, Garcia-Rovés P, Balboa E, Zanlungo S, Fabrías G, Casas J, Enrich C, Garcia-Ruiz C, Fernández-Checa JC (2016) Mitochondrial GSH replenishment as a potential therapeutic approach for Niemann Pick type C disease. Redox Biol 11:60-72.Human
Mouse
Nervous system
Liver
Endothelial;epithelial;mesothelial cell
Fibroblast
Other
Ejaz 2016 Diabetes2016Ejaz A, Martinez-Guino L, Goldfine AB, Ribas-Aulinas F, De Nigris V, Ribó S, Gonzalez-Franquesa A, Garcia-Roves PM, Li E, Dreyfuss JM, Gall W, Kim JK, Bottiglieri T, Villarroya F, Gerszten RE, Patti ME, Lerin C (2016) Dietary betaine supplementation increases Fgf21 levels to improve glucose homeostasis and reduce hepatic lipid accumulation in mice. Diabetes 65:902-12.MouseFatDiabetes
Kontro 2016 Thesis2016Kontro H (2016) Mitochondrial function and sirtuin expression in hippocampus of young and old high and low-capacity runner rats. Master's Thesis p35.RatNervous systemAging;senescence
Pokkunuri 2016 Oxid Med Cell Longev2016Pokkunuri I, Ali Q, Asghar M (2016) Grape powder improves age-related decline in mitochondrial and kidney functions in Fischer 344 rats. Oxid Med Cell Longev ID 6135319:8p.RatKidneyAging;senescence
MiPNet20.12 IOC103 Manaus2015-07-06
O2k-Network
Manaus BR, 2015 Jul 06-10. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.
MiPNet17.02 PBI-Shredder manual2015-06-19
O2k-Manual
PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.
MiPNet17.03 Shredder vs Fibers2015-06-18
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
FishesHeart
Liver
Canto 2015 Curr Protoc Mouse Biol2015Cantó C, Garcia-Roves PM (2015) High-resolution respirometry for mitochondrial characterization of ex vivo mouse tissues. Curr Protoc Mouse Biol 5:135-53.MouseHeart
Skeletal muscle
Nervous system
Liver
Fat
Burtscher 2015 Mitochondrion2015Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion 25:104-12.MouseNervous systemNeurodegenerative
Hasli 2015 Master Thesis2015Hasli PR (2015) Characterization of mitochondrial respiration and quality differences in diploid and triploid Atlantic salmon (Salmo salar L.) at 5 °C, 10 °C and 15 °C. Master Thesis:1-147.FishesHeart
Skeletal muscle
Temperature
Kluckova 2015 Methods Mol Biol2015Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94.MouseEndothelial;epithelial;mesothelial cell
Genital
Other cell lines
Cancer
Beauchamp 2015 Biosci Rep2015Beauchamp B, Thrush AB, Quizi J, Antoun G, McIntosh N, Al-Dirbashi OY, Patti ME, Harper ME (2015) Undernutrition during pregnancy in mice leads to dysfunctional cardiac muscle respiration in adult offspring. Biosci Rep 35. pii:e00200.MouseHeart
Liver
Cardiovascular
Diabetes
Obesity
Larsen 2014 Anal Biochem2014Larsen S, Kraunsøe R, Gram M, Gnaiger E, Helge JW, Dela F (2014) The best approach: homogenization or manual permeabilization of human skeletal muscle fibers for respirometry? Anal Biochem 446:64-8.Human
Mouse
Skeletal muscle
Gross 2011 Anal Biochem2011Gross VS, Greenberg HK, Baranov SV, Carlson GM, Stavrovskaya IG, Lazarev AV, Kristal BS (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Anal Biochem 418:213-23.RatSkeletal muscle
Kidney

List of abstracts: PBI-Shredder

 YearReferenceMammal and modelTissue and cellStressDiseases
Irving 2013 Abstract MiP20132013Irving BA, Stahl R, Kane CJ, Chernousov M, Argyropoulos G, Carey D (2013) Sex differences in murine mitochondrial oxidative capacity following a 24 week high-fat diet. Mitochondr Physiol Network 18.08.MouseSkeletal muscle
Liver
Fat
Obesity
Krumschnabel 2013 Abstract MiP20132013Krumschnabel G, Eigentler A, Fontana-Ayoub M, Draxl A, Fasching M, Gnaiger E (2013) Tissue homogenates for respiratory OXPHOS analysis in comparative mitochondrial physiology: mouse and trout – heart and liver. Mitochondr Physiol Network 18.08.Mouse
Fishes
Heart
Nervous system
Liver
Eigentler 2012 Abstract Bioblast2012Eigentler A, Draxl A, Fontana-Ayoub M, Gnaiger E (2012) The PBI-Shredder - an auxiliary HRR-tool for the preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.12.MouseHeart
Nervous system
Liver
PBI-Shredder SG3.jpg
PBI-Shredder HRR-Set.JPG
Shredder-Tube Cap Tool.JPG
Shredder-Tube Analyt Biochem.jpg