MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver: Difference between revisions
No edit summary |
Bader Helga (talk | contribs) No edit summary |
||
Line 19: | Line 19: | ||
|preparations=Homogenate | |preparations=Homogenate | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CI | |substratestates=CI, CII, CI&II | ||
|instruments=Oxygraph-2k, O2k-Manual, Protocol | |instruments=Oxygraph-2k, O2k-Manual, Protocol | ||
|additional=Mt-Preparation, PBI-Shredder | |additional=Mt-Preparation, PBI-Shredder |
Revision as of 16:20, 11 February 2015
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. |
ยป Open Access-MiPNet17.15.pdf; Versions
OROBOROS (2013-01) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15.
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข Keywords: Tissue homogenate, Shredder
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Mt-Preparation, PBI-Shredder