Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"
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|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] | ||
|authors= | |authors=Oroboros | ||
|year=2015-06-16 | |year=2015-06-16 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.''' »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | |abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.''' »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | ||
The [[O2k-Fluorescence LED2-Module]] is an extension of the | The [[O2k-Fluorescence LED2-Module]] is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | ||
:>> Product: [ | :>> Product: [[Oroboros O2k], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
:>> [[Permeabilized muscle fibres]] | :>> [[Permeabilized muscle fibres]] | ||
|mipnetlab=AT Innsbruck | |mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom | ||
|link= | |link= | ||
}} | }} | ||
Revision as of 10:23, 17 October 2017
{{Publication
|title=
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
|info=
|authors=Oroboros
|year=2015-06-16
|journal=Mitochondr Physiol Network
|abstract=Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6. »Versions
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> Product: [[Oroboros O2k], O2k-Catalogue
- >> Permeabilized muscle fibres
|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom |link= }}
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder
