Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"
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The [[O2k-Fluorescence LED2-Module]] is an extension of the OROBOROS O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | The [[O2k-Fluorescence LED2-Module]] is an extension of the OROBOROS O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k- | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
:>> [[Permeabilized muscle fibres]] | :>> [[Permeabilized muscle fibres]] | ||
|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck MitoCom | |mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck MitoCom | ||
Revision as of 14:24, 24 June 2015
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OROBOROS (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.
The O2k-Fluorescence LED2-Module is an extension of the OROBOROS O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> Product: OROBOROS O2k, O2k-Catalogue
- >> Permeabilized muscle fibres
• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder
