MiPNet18.16 IOC87 Spitsbergen
 Spitsbergen NO, 2014 Mar 24-27. Oroboros O2k-Workshop on HRR and O2k-Fluorometry: Spitsbergen - Enjoy the Cold, IOC87. MiPNet Lab »NO Trondheim Rognmo O. |
Oroboros (2014-03-24) Mitochondr Physiol Network
Abstract:
87th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry in cooperation with O2k-Network Lab NO Trondheim Rognmo O.
• O2k-Network Lab: NO Trondheim Rognmo O, AT Innsbruck Oroboros
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2014, O2k-Network, MitoCom
- Adaption and risk assessment of human activities in cold environments, Ny-Alesund, NO
- This course in the very unique and remote town of Ny-Alesund, NO was organized by the Cardiovascular Exercise and Research Group (CERG, head Ulrik Wisloff) - mainly by Svein Erik Gaustad and Andreas Møllerløkken from the NTNU University in Trondheim. An O2k-Workshop on HRR and Fluorometry (IOC87) was integrated into the course and held by Erich Gnaiger and Verena Laner.
- Dr. Erich Gnaiger gave a presentation entitled Mitochondrial respiratory function in the cold on March 26.
Lecturers and tutors
- Gnaiger Erich, CEO, Oroboros Instruments
- Laner Verena; Project manager and application specialist, Oroboros Instruments
Enjoy the cold
Adaption and risk assessment of human activities in cold environments, Ny-Alesund, NO
- This course in the very unique and remote town of Ny-Alesund was organized by the Cardiovascular Exercise and Research Group (CERG, head Ulrik Wisløff) - mainly by Svein Erik Gaustad and Andreas Møllerløkken from the NTNU University in Trondheim. The O2k-Workshop on HRR and Fluorometry (IOC87) was integrated into the course.
Waiting for departure at the Lufthavn in Longyearbyen. Due to bad weather, the original flight was cancelled.
First glimpse of the mountains of Svalbard. Very impressive: the untouched snow cover.
Outdoortraining part I with our instructor Johan Skullman (JOS eXperience AB): Learning how to make fire in stormy weather conditions.
Testing the immersion suits: Erich is ready for the arctic water.
Outdoor training part II: floating in an immersion suit for about 30 mins. For safety reasons, every person was secured by a rope, in this case Erich was held by Verena.
Ulrik Wisloff (CERG, NTNU University, Trondheim) and Erich after his swim in the arctic sea.
Monitoring of the body function after Thor Øyvind Hagen's (Aclima AS, NO) stay in the water for 60 mins by Ulrik Wisloff and Trine Karlsen (CERG, NTNU University, Trondheim). Everything was documented by photographer Roger Tangvik.
O2k-Workshop given by Erich with a real-time experiment on freeze-dried baker's yeast.
Assistance by Verena Laner.
Svein Erik Gaustad (main organizer, CERG, NTNU Trondheim, NO) and Atefe Tari (CERG) looking at the latest data from the tests.
The 'Enjoy the cold' group during a break.
Outdoor-training part III: Testing windsacks - Verena is recovering from the cold before and enjoying her time in the windsack.
Outdoor-training part IV: Building up your tent in stormy conditions. Andreas Møllerløkken (main organizer, CERG, NTNU, Trondheim, NO) and Verena after the successful installation by the group.
Instructor Johan talking about sleeping in a snow cave and preparing the participants for the field trip.
Anne-Sofie Burgaard Breistein and Bjørnar Heide (Petroleum Authority Safety, NO) in the field kitchen, boiling water for the group.
Chris Roos (Ministery of Defense, NL) and Martin Linnell after the night in their snow cave - happy and relaxed.
Group picture before the return to Ny-Alesund (left to right, back row first): Ulrik, Trine, Atefe, Johan, Erich and Verena.
Henrik Andersson (R&D Fjällräven, SE) and Johan Skullman (instructor) before the departure from Ny-Alesund.
Saying goodbye to Svalbard: Verena and Svein Erik Gaustad (CERG, NTNU University, Trondheim, NO).
Recommended reading
O2k-Core Manual
- New: »O2k-Core Manual.pdf
SUIT protocols for high-resolution respirometry
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access
HRR and O2k-Fluorometry
- »Manual: O2k-Fluo LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. »Open Access
- »O2k-Fluorometry
Mitochondrial pathways
- Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.
- O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
