MiPNet19.18D O2k-Series G and DatLab 6: Calibration: Difference between revisions
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|year=2013 | |year=2013 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''O2k-Manual Chapter D''' | |abstract='''O2k-Manual Chapter D''' ย | ||
[[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) | [[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) | ||
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procedure is described for O2k high-resolution respirometry with the | procedure is described for O2k high-resolution respirometry with the | ||
automatic calibration routine of DatLab. | automatic calibration routine of DatLab. | ||
:* [http://www.oroboros.at/?o2k-manual O2k-Manual contents] | |||
:* [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k] | |||
|keywords=HRR, DatLab | |keywords=HRR, DatLab | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS |
Revision as of 15:28, 16 October 2013
Gnaiger E (2013) Oxygen and pX (pH) Calibration by DatLab. Mitochondr Physiol Network 12.08. |
ยป Bioblast File:MiPNet12.08 O2k-Calibration.pdf, MiPNet12.08 Open Access
OROBOROS (2013) Mitochondr Physiol Network
Abstract: O2k-Manual Chapter D
Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.
โข Keywords: HRR, DatLab
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP, DatLab
Info
- Version 6: 2012-10-24
- Version 1: 2007
- MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) are integrated into MiPNet12.08.