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Difference between revisions of "MiPNet21.16 DatLab 7 Innovations"

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|authors=Oroboros
|authors=Oroboros
|year=2017-11-07
|year=2019-09-23
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Gradl L, Capek O, Doerrier C, Sigl R, Mészáros A, Gnaiger E (2017) DatLab 7: innovations. Mitochondr Physiol Network 21.16'''
|abstract=Doerrier C, Tindle-Solomon L, Gradl L, Gnaiger E (2019) DatLab 7.4: innovations. Mitochondr Physiol Network 21.16(03)
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]
'''DatLab 7''' is the newly developed software for [[High-Resolution_FluoRespirometry| High-Resolution FluoRespirometry (HRFR)]] incorporating the legacy of previous DatLab versions and new, user-friendly features on quality control, documentation, and traceability of measurements with the [[Oroboros O2k]].
'''DatLab 7.4''' is the software for [[high-resolution respirometry| high-resolution respirometry (HRR)]] incorporating the legacy of previous DatLab versions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, data analysis, documentation, and traceability of measurements with the [[Oroboros O2k]].


:» Product: [[DatLab]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]
:» Product: [[DatLab]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]
|keywords= DatLab, [[MiPNet22.16 DatLab 7.1 Innovations: DL-Protocols|Innovation DL-Protocols]]
|keywords= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]
|mipnetlab=AT_Innsbruck_Oroboros
|mipnetlab=AT_Innsbruck_Oroboros
}}
}}


== DatLab 7.4 Innovations ==


=== Oroboros O2k data acquisition ===
=== Quality control and traceability ===
==== User ====
:::: For quality control, DatLab 7.4 provides by default the option to choose/write a user code or name upon starting DatLab.
::::::: [[File:User DatLab.PNG|400px]]


:::: DatLab 7 is optimized for the new Oroboros O2k-Series H applications, and supports all configurations of the previous O2k-Series (A-G). At the heart of DatLab 7, a conceptual unification is obtained in calibrations and flux analysis of the [[O2k-FluoRespirometer]] channels (oxygen and fluorescence: H2O2, mitochondrial membrane potential, calcium, ATP) and add-on O2k-Modules (ion selective electrodes: TTP+ and pH; amperometric modules: nitric oxide or hydrogen suflide).
:::: For single user access to DatLab, the new DatLab 7.4 allows you to select the previous user.
:::::::::: [[File:Change user DatLab.PNG|300px]]


=== Display and graphs ===
==== Calibration and background source ====
:::: For increasing traceability, DatLab 7.4 incorporates the DLD file name of the calibrations and backgrounds applied.
:::::::::: [[File:Background_source.PNG|400px]]
 
=== Run DL-Protocol(DLP) ===
:::: DL-Protocols can be selected in DatLab 7.4 in the pull-down menu [Protocols]\Run DL-Protocol/Set O2 limit. A DL-Protocol defines the sequence of Events and Marks. By clicking on "Show" a demo DL-Protocol will be opened. Templates for storing exported data in a database and for data analysis are linked to DL-Protocols. A specific DL-Protocol can be assigned to Chamber A or B, or both.
::::» [[Run DL-Protocol/Set O2 limit]]
::::» [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols |Innovations: DL-Protocols]]
 
=== Lower O2 limit [μM] ===
:::: Lower O<sub>2</sub> limits optimized by the Oroboros MitoFit Laboratory are part of the DL-protocols provided. The lower O<sub>2</sub> limit can be individually set for each chamber and modified by the users. When the pre-set value is reached, an automatic WARNING is triggered to remind the user that re-oxygenation of the medium may be required.
:::: To modify the O2 limit, select [Protocols]\Run DL-Protocol\Set O2 limit, tick the box next to Lower O2 limit [µM], enter desired value and click on Apply to Chamber A or B, or both. DatLab 7.4 allows the user to set a default lower O<sub>2</sub> limit in a specific SUIT protocol by creating a [[Export DL-Protocol User (*.DLPU) |DL-Protocol User]]
::::» [[Run DL-Protocol/Set O2 limit]]
 
=== Synchronous DL-Protocol events ===
:::: If "Synchronous DL-Protocol event sets" is ticked, simultaneous events in both O2k-Chambers if the same DL-Protocol is assigned to both chambers.
 
=== Instrumental: Browse DL-Protocols and templates ===
:::: DatLab 7.4 incorporates a new collection of instrumental [[Run DL-Protocol/Set O2 limit |DL-Protocols]] and templates used for maintenance, calibrations and instrumental quality control for O<sub>2</sub>, AmR, Fluo and MgG applications, without experimental sample in the incubation medium. The user is guided stepwise through the protocol (displayed in the DL-Protocol window), facilitating calibration steps, minimizing handling errors and greatly improving performance during O2k measurements.
:::: Click on menu [Protocols]\Instrumental: Browse DL-Protocols and templates to open a folder with all the Instrumental protocols provided with the DatLab 7.4 installation. Example traces with instructions and brief explanatory texts and templates for data evaluation can be opened.


:::: Graphs are further improved for real-time display and publication. Versatile options allow an optimal setup of the graphical display of data. Standard graph layouts are provided. Lab and User-defined graph layouts can be stored and organized to support specific applications.
=== SUIT: Browse DL-Protocols and templates ===
:::: A comprehensive set of new [[substrate-uncoupler-inhibitor titration|SUIT]] protocols with example traces, including instructions, brief explanatory texts and templates for data evaluation can be browsed from inside DatLab 7.4.
:::: Click on menu [Protocols]\SUIT: Browse DL-Protocols and templates to open a folder with all SUIT protocols provided with the DatLab 7.4 installation. DL-Protocols for different [[Mitochondrial preparations |mitochondrial preparations]] and [[Living cells |living cells]]can be chosen to assess multiple sequences of respiratory coupling control and ET-pathway states. 
:::: The DL-Protocol window displays the sequence of pre-programmed [[Events - DatLab |events]] with chemicals, titration volumes, and corresponding final concentrations. These are saved automatically when the event is set. For DL-Protocols, specific [[Marks - DatLab |mark]] names are automatically assigned with titration volumes, used for calculation of sample dilution in the [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |O<sub>2</sub> analysis template DL7.4]]. DL-Protocols possess unique D### codes and comprise a fixed sequence of events and marks, which cannot be changed by the user in a DL-Protocol. However, with DatLab 7.4 the user has more flexibility and the fixed sequence of events and marks may be edited and/or changed (Skip/Added) in a SUIT protocol and saved as user-specific DL-Protocol [[Export DL-Protocol User (*.DLPU) |DLPU]].


=== Management of user accounts and experiments ===
=== Install Oroboros protocol package ===
:::: The standard '''Instrumental and SUIT DL-Protocols''' package is automatically implemented with the simple DatLab programme installation. We recommend a 'clean install': rename your previous DatLab programme subdirectory (''e.g.'' C:\DatLab_OLD).


:::: As a new feature, user accounts are assigned to each measurement for better traceability and customization. This facilitates the organization of experimental data and allows user-specific configurations in DatLab 7. A detailed description section, cohort, sample and subsample data in the “Sample” window improves organization and analysis of results.  
=== Browse www.bioblast.at/index.php./Mito-Pedia SUIT ===
:::: Link to the web with the library of SUIT protocols and updates is available.


=== DL-Protocols ===
=== SUITbrowser ===
:::: [[SUITbrowser]] is the new tool to find/design the best substrate-uncoupler-inhibitor-titration (SUIT) protocol for your research questions.


:::: Besides keeping the flexibility to run user-defined experiments, DatLab 7 is now equipped with the new [[DL-Protocols]] feature. A collection of evaluated and tested DL-Protocols are available providing instructions for performing standardized experiments according to a selected [[substrate-uncoupler-inhibitor titration]] (SUIT) protocol or instrumental quality control protocols (calibration and instrumental background tests). The user is guided stepwise through the protocol (displayed in the DL-Protocol window), facilitating calibration steps, minimizing handling errors and greatly improving performance during O2k measurements. Moreover, an automatic warning at low oxygen levels can be set to remind the user to re-oxygenate the chamber in time. The DL-Protocol window displays the sequence of pre-programmed [[Events - DatLab |events]] with chemicals, titration volumes and corresponding final concentrations. These are saved automatically when the event is set. For protocol-specific [[Marks - DatLab |marks]] the mark names are automatically assigned, with titration volumes which are used for calculation of sample dilution in analysis templates.
=== Enable DL-Protocol editing and Export DL-Protocol User (*.DLPU) ===
:::: DatLab 7.4 offers a new feature in DL-Protocols: flexibility. Fixed sequence of events and marks can be changed (Skip/Added) in a SUIT protocol by the user. Moreover, editions of text, instructions, concentrations and titration volumes of injections in a specific DL-Protocol can be edited and saved as DL-Protocol User (*.DLPU).
::::» [[Enable DL-Protocol editing]]
::::» [[Export DL-Protocol User (*.DLPU)]]


=== Events and marks ===


:::: Concentration and volume of the chemicals added during [[MitoPedia:_SUIT |'''Substrate-uncoupler-inhibition-titration (SUIT)''']] protocols can be stored together with event names and comments. Automatic outlier detection helps to avoid unwanted data points when setting marks during respiratory steady-states. On top the user has the option to delete, interpolate or restore data points of marks.  
=== Oxygen flux analysis template for DatLab 7.4 ===
:::: DatLab 7.4 provides a new Excel template for time-efficient data analysis.
::::» [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |O<sub>2</sub> analysis template DL7.4]]


=== Mark statistics ===
=== Mark statistics ===
:::: Numerous statistical functions are available for data analysis. “Marks statistics” organizes the marks and provides flexibility in displaying DL-Protocol marks, user-defined marks or both. The copy-to-clipboard function, with extended settings for backward compatibility, facilitates export to any third-party analysis software.
=== Compatible with all O2k series ===
:::: DatLab 7.4 is optimized for the new Oroboros O2k-Series H and supports all configurations of the previous O2k-Series.
=== O2k channel labels ===
:::: Default channel labels can now be changed, and new labels set by the user. E.g., rename the Amperometric channel, Amp, to 'H2O2' for H<sub>2</sub>OH<sub>2</sub>O measurements by fluorometry; rename the potentiometric channel, pX, to TPP+ for mitochondrial membrane measurements with the O2k-pH ISE-Module.
:::: For changing the label, go to menu [Oroboros O2k]\O2k channel labels and set the new channel label as desired.


:::: Numerous statistical functions are available for data analysis. “Marks statistics” organizes the marks and provides flexibility in displaying DL-Protocol marks, user-defined marks or both. The copy-to-clipboard function, with extended settings for backward compatibility, facilitates export to any third-party analysis software.  
=== Display and graphs ===
:::: Graphs are further improved for real-time display and publication. Versatile options allow for an optimal setup of the graphical display of data. Standard graph layouts are provided. Lab and User-defined graph layouts can be stored and organized to support specific applications.


=== Experimental log ===
=== Management of user accounts and experiments ===
:::: As a new feature, user accounts are assigned to each measurement for better traceability and customization. This facilitates the organization of experimental data and allows user-specific configurations in DatLab 7. A detailed description section, cohort, sample and subsample data in the “Sample” window improves organization and analysis of results.


:::: An [[Experimental_log_-_DatLab |Experimental log]] enables to view and generate detailed report of experimental-, software- and hardware settings for optimal tracking of each experiment.
=== Events and marks ===
:::: Concentration and volume of the chemicals added during [[MitoPedia:_SUIT |'''SUIT''']] protocols can be stored together with event names and comments. Automatic outlier detection helps to avoid unwanted data points when setting marks during respiratory steady-states. The user has the option to delete, interpolate or restore data points of marks.  


=== Contextual help ===
=== Contextual help ===
 
:::: A hyperlink-based contextual help system provides optimal support throughout settings and experimental steps in DatLab 7. [[MitoPedia:_DatLab |'''MitoPedia''']] provides the background for this function, which guarantees up-to-date and verified information anytime.
:::: A hyperlink-based contextual help system provides optimal support throughout settings and experimental steps in DatLab 7. [[MitoPedia:_DatLab |'''MitoPedia''']] provides the background for this function, which warranties up-to-date and verified information anytime.


=== TIP2k control ===
=== TIP2k control ===
:::: The software-driven, feedback-controlled [[TIP2k-Module |Titration-Injection microPump (TIP2k)]] is an integral part of the O2k. In DatLab 7 TIP2k control has improved and facilitates user friendly and more flexible programming.
[[File:O2k-Concept.jpg|right|300px |O2k-Concept]]


:::: The software-driven, feedback-controlled [[TIP2k-Module |Titration-Injection microPump (TIP2k)]] is an integral part of the O2k. In DatLab 7 TIP2k control has improved and facilitates user friendly and more flexible programming.


[[File:DatLab-bar.jpg|460px|link=DatLab]]
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]
<big><big>'''[[DatLab installation]]'''</big></big>




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|additional=O2k-Core, DatLab, DL7
|additional=O2k-Core, DatLab, DL7
}}
}}
[[File:O2k-Fluorometer.JPG|200 px|right|link=O2k-Fluorometer|O2k-Fluorometer]]
» [[O2k-Protocols]]

Revision as of 13:01, 6 March 2020

                



MiPNet21.16 DatLab 7 Innovations

Publications in the MiPMap
About Oroboros
DatLab 7: innovations.


Oroboros (2019-09-23) Mitochondr Physiol Network

Abstract: Doerrier C, Tindle-Solomon L, Gradl L, Gnaiger E (2019) DatLab 7.4: innovations. Mitochondr Physiol Network 21.16(03)

DatLab

DatLab 7.4 is the software for high-resolution respirometry (HRR) incorporating the legacy of previous DatLab versions and new, user-friendly features on DL-protocols, DL-Protocol User, quality control, data analysis, documentation, and traceability of measurements with the Oroboros O2k.

» Product: DatLab, Oroboros O2k, O2k-Catalogue

Keywords: DatLab, Innovation DL-Protocols

O2k-Network Lab: AT_Innsbruck_Oroboros


DatLab 7.4 Innovations

Quality control and traceability

User

For quality control, DatLab 7.4 provides by default the option to choose/write a user code or name upon starting DatLab.
User DatLab.PNG
For single user access to DatLab, the new DatLab 7.4 allows you to select the previous user.
Change user DatLab.PNG

Calibration and background source

For increasing traceability, DatLab 7.4 incorporates the DLD file name of the calibrations and backgrounds applied.
Background source.PNG

Run DL-Protocol(DLP)

DL-Protocols can be selected in DatLab 7.4 in the pull-down menu [Protocols]\Run DL-Protocol/Set O2 limit. A DL-Protocol defines the sequence of Events and Marks. By clicking on "Show" a demo DL-Protocol will be opened. Templates for storing exported data in a database and for data analysis are linked to DL-Protocols. A specific DL-Protocol can be assigned to Chamber A or B, or both.
» Run DL-Protocol/Set O2 limit
» Innovations: DL-Protocols

Lower O2 limit [μM]

Lower O2 limits optimized by the Oroboros MitoFit Laboratory are part of the DL-protocols provided. The lower O2 limit can be individually set for each chamber and modified by the users. When the pre-set value is reached, an automatic WARNING is triggered to remind the user that re-oxygenation of the medium may be required.
To modify the O2 limit, select [Protocols]\Run DL-Protocol\Set O2 limit, tick the box next to Lower O2 limit [µM], enter desired value and click on Apply to Chamber A or B, or both. DatLab 7.4 allows the user to set a default lower O2 limit in a specific SUIT protocol by creating a DL-Protocol User
» Run DL-Protocol/Set O2 limit

Synchronous DL-Protocol events

If "Synchronous DL-Protocol event sets" is ticked, simultaneous events in both O2k-Chambers if the same DL-Protocol is assigned to both chambers.

Instrumental: Browse DL-Protocols and templates

DatLab 7.4 incorporates a new collection of instrumental DL-Protocols and templates used for maintenance, calibrations and instrumental quality control for O2, AmR, Fluo and MgG applications, without experimental sample in the incubation medium. The user is guided stepwise through the protocol (displayed in the DL-Protocol window), facilitating calibration steps, minimizing handling errors and greatly improving performance during O2k measurements.
Click on menu [Protocols]\Instrumental: Browse DL-Protocols and templates to open a folder with all the Instrumental protocols provided with the DatLab 7.4 installation. Example traces with instructions and brief explanatory texts and templates for data evaluation can be opened.

SUIT: Browse DL-Protocols and templates

A comprehensive set of new SUIT protocols with example traces, including instructions, brief explanatory texts and templates for data evaluation can be browsed from inside DatLab 7.4.
Click on menu [Protocols]\SUIT: Browse DL-Protocols and templates to open a folder with all SUIT protocols provided with the DatLab 7.4 installation. DL-Protocols for different mitochondrial preparations and living cellscan be chosen to assess multiple sequences of respiratory coupling control and ET-pathway states.
The DL-Protocol window displays the sequence of pre-programmed events with chemicals, titration volumes, and corresponding final concentrations. These are saved automatically when the event is set. For DL-Protocols, specific mark names are automatically assigned with titration volumes, used for calculation of sample dilution in the O2 analysis template DL7.4. DL-Protocols possess unique D### codes and comprise a fixed sequence of events and marks, which cannot be changed by the user in a DL-Protocol. However, with DatLab 7.4 the user has more flexibility and the fixed sequence of events and marks may be edited and/or changed (Skip/Added) in a SUIT protocol and saved as user-specific DL-Protocol DLPU.

Install Oroboros protocol package

The standard Instrumental and SUIT DL-Protocols package is automatically implemented with the simple DatLab programme installation. We recommend a 'clean install': rename your previous DatLab programme subdirectory (e.g. C:\DatLab_OLD).

Browse www.bioblast.at/index.php./Mito-Pedia SUIT

Link to the web with the library of SUIT protocols and updates is available.

SUITbrowser

SUITbrowser is the new tool to find/design the best substrate-uncoupler-inhibitor-titration (SUIT) protocol for your research questions.

Enable DL-Protocol editing and Export DL-Protocol User (*.DLPU)

DatLab 7.4 offers a new feature in DL-Protocols: flexibility. Fixed sequence of events and marks can be changed (Skip/Added) in a SUIT protocol by the user. Moreover, editions of text, instructions, concentrations and titration volumes of injections in a specific DL-Protocol can be edited and saved as DL-Protocol User (*.DLPU).
» Enable DL-Protocol editing
» Export DL-Protocol User (*.DLPU)


Oxygen flux analysis template for DatLab 7.4

DatLab 7.4 provides a new Excel template for time-efficient data analysis.
» O2 analysis template DL7.4

Mark statistics

Numerous statistical functions are available for data analysis. “Marks statistics” organizes the marks and provides flexibility in displaying DL-Protocol marks, user-defined marks or both. The copy-to-clipboard function, with extended settings for backward compatibility, facilitates export to any third-party analysis software.

Compatible with all O2k series

DatLab 7.4 is optimized for the new Oroboros O2k-Series H and supports all configurations of the previous O2k-Series.

O2k channel labels

Default channel labels can now be changed, and new labels set by the user. E.g., rename the Amperometric channel, Amp, to 'H2O2' for H2OH2O measurements by fluorometry; rename the potentiometric channel, pX, to TPP+ for mitochondrial membrane measurements with the O2k-pH ISE-Module.
For changing the label, go to menu [Oroboros O2k]\O2k channel labels and set the new channel label as desired.

Display and graphs

Graphs are further improved for real-time display and publication. Versatile options allow for an optimal setup of the graphical display of data. Standard graph layouts are provided. Lab and User-defined graph layouts can be stored and organized to support specific applications.

Management of user accounts and experiments

As a new feature, user accounts are assigned to each measurement for better traceability and customization. This facilitates the organization of experimental data and allows user-specific configurations in DatLab 7. A detailed description section, cohort, sample and subsample data in the “Sample” window improves organization and analysis of results.

Events and marks

Concentration and volume of the chemicals added during SUIT protocols can be stored together with event names and comments. Automatic outlier detection helps to avoid unwanted data points when setting marks during respiratory steady-states. The user has the option to delete, interpolate or restore data points of marks.

Contextual help

A hyperlink-based contextual help system provides optimal support throughout settings and experimental steps in DatLab 7. MitoPedia provides the background for this function, which guarantees up-to-date and verified information anytime.

TIP2k control

The software-driven, feedback-controlled Titration-Injection microPump (TIP2k) is an integral part of the O2k. In DatLab 7 TIP2k control has improved and facilitates user friendly and more flexible programming.
O2k-Concept


DatLab-bar.jpg

DatLab
DatLab installation


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Protocol 

O2k-Core, DatLab, DL7