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MiPNet23.11 IOC137 Innsbruck AT

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MiPNet23.11 IOC137 Innsbruck AT

Publications in the MiPMap
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Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC.


Oroboros (2018-12-10) Mitochondr Physiol Network

Abstract:

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MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Dec 10-12.


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


Labels:






ORO, IOC, 2018, MitoFit, TRANSMIT, Next, MitoGlobal 




General information

TRANSMIT
REMIX
MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
Early stage researchers (ESR´s) during their Marie Skłodowska-Curie project - TRANSMIT or REMIX will be trained at the O2k-Workshop on High-Resolution FluoRespirometry:
» https://www.remix-itn.eu
» https://www.transmit-project.eu
» Information on travel and venue: IOC Innsbruck.
Please bring your labcoat with you. Thank you!

Registration

will open soon

Tutors, lecturers, and managers


Programme

Monday 10th Topic Room
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab
09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab
10:10 Hands-on: DatLab 7 ORO-MitoFit Lab
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 11th Topic Room
09:00 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
09:15 Application of the O2k Series H ORO-MitoFit Lab
09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:30 Introduction to DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Wednesday 12th Topic Room
09:00 MitoFit proficiency test:

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
10:00 Coffee
10:30 MitoFit proficiency test:

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:15 DatLab analysis ORO-MitoFit Lab
14:00 DatLab analysis summary ORO-MitoFit Lab
15:00 Coffee
15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt
16:15 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Abla Houda University of Bologna, Bologna (IT)
Participant Di Paola Floriana Jessica Justus-Liebig-University of Giessen, Giessen (DE)
Participant Saharnaz Sarlak FR Bordeaux Rossignol R - University of Bordeaux, Bordeaux (FR)
Participant Thapa Maheshwor AT Salzburg Sperl W - Biocrates Life Sciences AG, Innsbruck (AT)


Contact

[email protected]


Oroboros Instruments
High-Resolution FluoRespirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
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IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.