MitoEAGLE

MitoGlobal first

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MitoEAGLE

Mitochondrial mapping: Evolution - Age - Gender - Lifestyle - Environment

COST Action proposal: MITOEAGLE

Updates

• 2015-02-27 draft 02: Generalization from humans and mouse models to humans and rodent models; COST Action MITOEAGLE.
• 2015-02-24 draft 01: Invitation to participants, COST Action mtEAGLE.

Summary

The objective of the MITOEAGLE network is to improve our knowledge on mitochondrial function in health and disease related to Evolution, Age, Gender, Lifestyle and Environment. Every study of mitochondrial (mt) function and disease is faced with EAGLE as the essential background conditions characterizing the individual patient, subject, study group, species, tissue or even cell line. To address the complex interrelationships of EAGLE with an initial focus on humans and rodent models, the network will enhance the value of each individual study by starting to analyse and catalog data beyond the published record. Highlighting the topic of gender and mitochondrial function, unique new information will emerge on human biology from the development of a European reference database. Protocols, technologies and standard procedures will be compared and strategies defined for improvement of quality control. An inter-laboratory ring test will be established as a world-wide innovation in the field of mitochondrial respiratory physiology. The expertise gained and new standards developed will be integrated into a strategic dissemination and education programme for mitochondrial phenotyping, aiming at an expanding European and MitoGlobal EAGLE network where researchers collaborate on mapping mitochondrial physiology and medicine, complementary to established mtDNA databases.

Further details » MitoGlobal EAGLE

Working groups (WG)

WG1. MITOEAGLE data base on gender and mt-function in health and disease

• Mainly published data from WG participants will be shared in the detail beyond the published record (anonymised data on individuals in addition to group averages; tabulated results in addition to figures; conversion to common SI units; comparability of normalization).
• Measures of mitochondrial respiratory function in human and rodent tissues, blood cells and cell lines will be structured according to the EAGLE background, with specific emphasis on gender-specific mt-function and gender medicine.

WG2. Challenges on quality control for reference data in mitochondrial physiology

• Basic concepts and communication: Consolidation of concepts and nomenclature on mitochondrial respiratory states and control parameters; joint publication.
• Laboratory standards: Protocols, technologies and standard procedures; data base on laboratory protocols.
• Reference data in mitochondrial physiology: Quality control, documentation, bioinformatics standards.

WG3. Inter-laboratory test and outreach

• An interlaboratory ring test will be introduced in practice as a world-wide innovation in the field of mitochondrial respiratory physiology: design and implementation; reference sample dissemination; test protocol(s); data analysis; joint publication.
• Comparison of results between and within instrumental platforms.
• Logistics for extended future collaboration in Europe, North and South America, Asia, Australia and Africa.

WG4. Training and data sharing

• Recommendations for quality control, data reporting and data sharing beyond the published record.
• Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
• Summary on strategic dissemination and education programme for MitoGlobal EAGLE.

Objective

Mitochondria play multiple roles in health and degenerative diseases. To understand these roles better is a current need in the European research, medical and commercial communities, particularly to implement protective and preventive measures optimized according to age, gender, lifestyle (nutrition, sport), and environment. In addition, results on humans and comparison with animal models requires evaluation of the evolutionary background beyond the scope of each individual study or research group. Therefore, the MITOEAGLE network aims at mapping data on mitochondrial function as a basis of better understanding the complex background of EAGLE. Strategies will be implemented to improve standardized methods for evaluating and scoring acute and latent mitochondrial dysfunction, at baseline and in response pathological stress. As a result, interventions (nutrition, exercise, drugs, novel treatments) that potentially impact on mitochondrial health, can be evaluated objectively in different labs and health-care institutions. By coordinating a critical mass of expertise, the network will address the problems that may have prevented so far the major break-though required for relating the quality of life and the severity of disease to a clinically standardized measure of mitochondrial function – providing vital knowledge to improve health.

Keywords

mitochondrial physiology, gender, aging, oxidative stress, life style, nutrition, sports, degenerative diseases, neurodegeneration, obesity, diabetes, cardiovascular diseases, cancer, latent mitochondrial dysfunction, stress resistance, respirometry, membrane potential, ROS production, redox regulation, isolated mitochondria, homogenate, cells, permeabilized cells, muscle fibres, tissues, model organisms

• MITOEAGLE aims at meeting current demands of the global research community to improve the data base on mitochondrial function/dysfunction in various tissues of humans and animal models, and to diagnose the mitochondrial implications in various pathologies, for development and evaluation of mitochondria-targeted therapies.
• MITOEAGLE opens the feedback loop between the primary problems of attack (gender-specific mitochondrial roles in fitness, aging, pathologies) and the consequential models and methods employed.
• MITOEAGLE will generate the critical mass of partners required to address the complex conceptual and methodological problems that may have prevented so far the major break-though required for addressing the preventable diseases in westernized ageing societies.
• MITOEAGLE is an initiative towards a broad but focussed project, to ‘contribute substantially to the coordination and defragmentation of research efforts across Europe’.

COST info

Participants

A participant of a COST Action means any researcher, engineer or scholar who participates actively in a COST Action.

» List of participants

Proposer

The main proposer of a COST Action means the coordinator of the network of proposers who submit a proposal for a COST Action in response to the open call. MITOEAGLE is an initiative of MitoCom. If you are interested to participate in the MITOEAGLE network, please contact:

Erich Gnaiger, Ao.Univ.-Prof., Ph.D.

Medical University of Innsbruck

Department of Visceral, Transplant and Thoracic Surgery

D. Swarovski Research Laboratory

A-6020 Innsbruck, Austria

Email: [email protected]

Link: MitoGlobal_EAGLE

Disclosure: Dr Erich Gnaiger is founder and CEO of OROBOROS INSTRUMENTS – www.oroboros.at

General

» Website COST Actions

» Current COST Actions in Biomedicine and Molecular Biosciences

COST member states

» 35 member states:

Austria, Belgium, Bosnia and Herzegovina, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom and the former Yugoslav Republic of Macedonia.

• COST cooperating state: Israel.