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Difference between revisions of "MitoEAGLE data: muscle"

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::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
::::# ''Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project.'' - '''[[Coen PM|Paul M Coen]]''' (2016).
::::# ''Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project.'' - '''[[Coen PM|Paul M Coen]]''' (2016).
::::# ''In particular, my team can participate with studies about the  mitochondrial function in relation to the supramolecular organization of  the respiratory chain where the dynamic presence of the respiratory  supercomplexes  is a way to shift between the main metabolic fluxes of  NAD- and FAD-dependent substrates in different metabolic conditions of  the cell. Besides my basic studies on the enzyme interactions with the  Coenzyme Q pool, I recently joined an Italian consortium of University  laboratories whose target is to shed light on the molecular mechanisms  underpinning muscle weakness and reduced muscle mass (sarcopenia) in  ageing and attenuated response to exercise training stimuli in the  elderly; both animal models and human subjects will be analyzed.'' - '''[[Genova ML| Maria Luisa Genova]]''' (2016).
 
== Projects in progress ==
== Projects in progress ==
::::» [[MiPMap]]
::::» [[MiPMap]]


=== Next steps ===
=== Next steps ===

Revision as of 07:37, 23 May 2016


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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE data: muscle



WG2

MITOEAGLE data repository in muscle and other tissues


MITOEAGLE Working Groups

WG2 Description

During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked.
The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types.

WG2 Participants

CountryCityContact
AustraliaMelbourneBishop David J
AustriaSalzburgMayr JA
AustriaInnsbruckDoerrier Carolina
AustriaInnsbruckLaner Verena
AustriaInnsbruckGnaiger Erich
BelgiumLiegeVotion Dominique-Marie
BelgiumLeuvenBird Matthew
BrazilSĂŁo PauloFerreira Julio Cesar B
BrazilJaboticabal, SPGarcia Geovana S
CanadaAntigonishKane Daniel A
CanadaRimouskiVandermeeren E
CanadaVancouverBoushel Robert C
CanadaEdmonton (Alberta)Lemieux Helene
CanadaRimouskiBlier Pierre U
CroatiaZagrebSaric A
CroatiaSplitMihanovic I
Czech RepublicPilsenKuncova J
Czech RepublicPragueElkalaf Moustafa
Czech RepublicHradec KrålovéWaldauf P
Czech RepublicPilsenMarkova Michaela
Czech RepublicPrague 10Krajcova Adela
DenmarkCopenhagenLarsen Steen
DenmarkHvidovreNehlin Jan O
DenmarkCopenhagenGonzalez-Franquesa Alba
DenmarkMalovHey-Mogensen M
DenmarkCopenhagenDela Flemming
DenmarkCopenhagen NMortensen OH
EstoniaTallinnAid-Vanakova Jekaterina
EstoniaTallinnTepp Kersti
EstoniaTallinnKarro N
EstoniaTartuPaju Kalju
EstoniaTallinnVendelin Marko
EstoniaTallinnPuurand Marju
EstoniaTallinnTruu Laura
EstoniaTallinnKaambre Tuuli
FinlandHelsinkiPirinen E
FinlandJyvaeskylaeLehti Maarit
FranceMontpellier Cedex 1Chabi Beatrice
FranceMontpellierCortade F
FranceParisVentura-Clapier R
FranceParisArmand Anne-Sophie
FranceParisPetit Patrice X
FranceBordeaux cedexMourier A
FranceGrenoble Cedex 9Schlattner Uwe
FranceGrenoble cedex 9Dubouchaud Herve
GermanyRostockHaider F
GermanyNuthetalKlaus S
GermanyMunichJastroch M
GermanyKölnKimoloi Sammy
GermanyBochumPlaten P
GermanyDuesseldorfRoden Michael
GermanyDĂŒsseldorfGranata Cesare
GermanyDuesseldorfJelenik T
GermanyBremerhavenMark Felix Christopher
GermanyLeipzigOst Mario
GermanyColognePesta Dominik
GreeceHeraklionTavernarakis Nektarios
GreeceHeraklioSidiropoulou K
GreeceAthensTrougakos Ioannis P
IndiaHaryanaSharma Vipin
IrelandDublinO'Gorman Donal
IrelandDublinPorter Richard K
IrelandSligoMurphy J
IsraelRishon Le ZionHachmo Yafit
IsraelZrifinSova Marina
IsraelJerusalemSaada Reisch Ann
ItaliaUdineSalvadego Desy
ItalyRomeScatena Roberto
ItalyMilanDe Palma Clara
ItalyMilanGiovarelli Matteo
ItalyPisaGastaldelli Amalia
ItalyBolognaGenova Maria Luisa
ItalyNovaraReano Simone
JapanSapporoNambu H
JapanSapporoMaekawa S
JapanSapporoShirakawa R
LatviaRigaInashkina I
LatviaRigaLiepins Edgars
LatviaRigaKuka Janis
LatviaRigaZvejniece Liga
LuxembourgStrassenDevaux Yvan
MaltaMsidaVella Joanna
NetherlandsMaastrichtVan Polanen N
NetherlandsAmsterdamDe Goede Paul
NetherlandsBT AmsterdamJaspers Richard T
NetherlandsMaastrichtHoeks Joris
NetherlandsMaastrichtGrevendonk L
NetherlandsWageningenKeijer Jaap
NiederlandeER MasstrichtDe Wit-Verheggen V
NiederlandeER MasstrichtDe Theije C
NorwayBergenTronstad Karl Johan
NorwayTromsoeSchots Pauke C
NorwayUllevÄlHeiestad Christina
NorwayTromsoeLarsen Terje S
NorwayOsloLund Jenny
NorwayOsloTorp May-Kristin
NorwayTromsoeAasum E
NorwayTromsoeJansen Kirsten M
NorwayOsloNikolic N
NorwayOsloRustan AC
NorwayOsloEide L
PolandWarsawDymkowska Dorota
PolandLodzWatala Cezary
PolandWarsawSek Aleksandra
PolandLodzLabieniec-Watala Magdalena
PolandWarsawKampa Rafal Pawel
PortugalCoimbraPalmeira Carlos
PortugalPortoMagalhaes Jose
PortugalCoimbraPereira Susana P
PortugalCoimbraCarvalho Eugenia
PortugalPortoBeleza Jorge
PortugalCantanhedeMartins Joao D
PortugalCantanhedeFerreira LL
PortugalCoimbraMartins Marques T
PortugalPortoCoxito Pedro
RomaniaTimisoaraLighezan R
RomaniaTimisoaraMuntean Danina M
SingaporeSingaporeHausenloy Derek J
SlovakiaBratislavaUkropcova B
SlovakiaBratislavaMaderova D
SlovakiaBratislavaNemec Michal
SlovakiaBratislavaUkropec J
SlovakiaBratislavaSumbalova Zuzana
SloveniaLjubljanaPirkmajer Sergej
SloveniaLjubljanaDolinar K
SloveniaLjubljanaMars T
South AfricaSmith James
SpainMadridCadenas S
SpainBarcelonaDe la Torre Lara J
SpainSevillaBustos Matilde
SpainMadridMoran M
SpainBarcelonaSebastian D
SpainBarcelonaBatlle M
SpainArmilla (Granada)Fernandez-Ortiz Marisol
SpainBarcelonaZorzano Antonio
SpainBarcelonaCatalan M
SpainArmilla (Granada)Acuna-Castroviejo Dario
SpainBarcelonaGarcia-Roves Pablo Miguel
SpainBarcelonaGama Perez Pau
SpainArmilla, GranadaEscames Germaine
SpainA CoruñaBlanco FJ
SpainBarcelonaGuitart M
SpainGranadaLopez Garcia Luis Carlos
SwedenStockholmCardinale Daniele A
SwedenLinköpingFontes-Oliveira CC
SwitzerlandLausanneGebara E
SwitzerlandBernNuoffer Jean-Marc
SwitzerlandLausanneMorato Fornaguera L
SwitzerlandLausanneSandi Carmen
SwitzerlandLausanneWeger M
SwitzerlandLausanneZalachoras I
The NetherlandsBT AmsterdamWuest Rob CI
The NetherlandsWageningenVan der Ende Miranda
TurkeyAnkaraTuncay Erkan
USATorranceRossiter Harry B
USAFort CollinsChicco Adam J
USAColorado SpringsJacobs RA
USANorfolkLai Nicola
USAGainesvilleHepple Russell T
USASeattleMarcinek DJ
USABoca RatonKhamoui Andy V
USABaltimoreFang Evandro F
USAAnn ArborBeard Daniel A
USAOrlandoDistefano Giovanna
USABaton RougeAxelrod Christopher L
USALittle RockPorter Craig
USASt LouisWelch R
USAChicagoDayanidhi Sudarshan
USAGainesvilleWohlgemuth Stephanie E
USAGainesvilleNeyroud D
USACollege StationLatham C
USAFairbanksO'Brien Kristin
USABaltimoreGonzalez-Freire Marta
USACollege StationWhite-Springer Sarah H
USAOrlandoCoen Paul M
USABaton RougeIrving Brian A
United KingdomCambridgeShiroma Jonathan G
United KingdomBrightonMoore Anthony L
United KingdomCambridgeMurray Andrew James
United KingdomBirminghamThakker A
United KingdomLeedsBoyle JP
United KingdomNottinghamChakrabarti Lisa
United KingdomBirminghamAshcroft S
United KingdomOxfordMakrecka-Kuka Marina
United KingdomBirminghamJoanisse S
United KingdomManchesterGiakoumaki I
United KingdomLoughboroughEbanks Brad
United KingdomBirminghamGarten Antje
United KingdomPlymouth, DevonMcKenna Helen T
United KingdomLondonSzabadkai Gyorgy
United StatesProvidenceSiamwala J


WG2 Management

Tasks

  1. Call for participating labs to deliver relevant experimental data on already published studies and to report them in a standardized format.
  2. Collection and indexing of the data sets as required for statistical analysis.
  3. Discussion of procedures, protocols and general guidelines.
  4. Draft a comprehensive review on mt-function in muscle tissue (registration at PROSPERO, link with EQUATOR).
  5. Opening of the database to the research public for data search and moderated entry of new data sets.


Milestones

  1. Call for data report delivered.
  2. Data sets entered in standardized format.
  3. Summary, interpretation and discussion of data for review.


Deliverables

  1. A MITOEAGLE database on muscle tissue from humans and model organisms.
  2. A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue.
  3. Draft of review manuscript.

WG2 Feedback

  1. Skeletal muscle is the main tissue we are studying in the lab. - Beatrice Chabi (2016).
  2. For the kind of work group, our expertise is certainly most on muscle tissue (skeletal and heart), although we also worked on all kinds of permeabilized cells (primary cells and cell lines). For all these, we have extended data sets, mainly rat, but also human, from diverse experimental setups (nutritional regimen, exercise, drugs ...). - Uwe Schlattner (2016).
  3. I would fit rather in WG1, as myself I am not involved in human muscle fat or blood. However, in our team we made a study involving human liver samples. - Frédéric Bouillaud (2016).
  4. In my working group we are mainly interested in muscle, especially focusing on aging and sarcopenia (using animal models). I would be happy if new cooperations could be formed through the COST Action. I could imagine working on a joint review article. Next to examining muscle from mouse models, we are studying biopsies from human heart muscle and liver, but there is no published data on these studies yet. - Susanne Klaus (2016). Translated by Sandra Fleischmann
  5. I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).
  6. I am working in the field of mitochondrial biochemistry since many years. In detail my coworkers and I are carrying out studies on the regulation of ATP synthase and respiratory complexes in swine heart mitochondria as biological model of mammalian mitochondria. Some comparative aspects of mitochondrial bioenergetics and ATP synthase responses are also investigated in bivalve mollusk mitochondria. - Alessandra Pagliarani (2016).
  7. We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
  8. My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
  9. We mostly work with cultured human skeletal muscle cells, from different donor groups such as lean, obese, type 2 diabetics, as well as muscle cells from athletes. We are interested in collaboration with our cells and measurements on energy metabolism and mitochondral function. - Arild Rustan (2016).
  10. Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
    1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
    2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
    3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
  11. I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
  12. As you know, I have received the MC nomination from our CNC in Bucharest (Ms Elena Dinu) for my colleague, Rodica Lighezan and myself. My expertise is most on muscle tissue (in particular, heart) and also, liver - so I will go for WG2 if this is fine with you. However, Both my colleague and I are mostly interested in performing respirometry in blood cells (and this is what we will start to try in the near future); thus, you decide whether we can fit both of us in WG4, or only her. Also, in order to extend the Network in other Romanian cities with powerful state medical universities, Cluj-Napoca (as you know) and Tg Mures - 2 young men, Bela Kiss and Ovidiu Cotoi have been proposed as substitute members (for gender balance too). In particular, we want to jointly ”explore” the exciting fields of mitochondrial ”hidden toxicity” and dynamics, respectively since the former colleague is a toxicologist and the latter a pathologist. I wish you good luck and I am indeed happy and proud to be part of this growing network. - Danina M Muntean (2016).
  13. I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
  14. Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
  15. In particular, my team can participate with studies about the mitochondrial function in relation to the supramolecular organization of the respiratory chain where the dynamic presence of the respiratory supercomplexes is a way to shift between the main metabolic fluxes of NAD- and FAD-dependent substrates in different metabolic conditions of the cell. Besides my basic studies on the enzyme interactions with the Coenzyme Q pool, I recently joined an Italian consortium of University laboratories whose target is to shed light on the molecular mechanisms underpinning muscle weakness and reduced muscle mass (sarcopenia) in ageing and attenuated response to exercise training stimuli in the elderly; both animal models and human subjects will be analyzed. - Maria Luisa Genova (2016).

Projects in progress

» MiPMap

Next steps