MitoKit-CII MitoPedia: Difference between revisions
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* [[SUIT-003_O2_ce_D060]] for living cells using [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]]. | * [[SUIT-003_O2_ce_D060]] for living cells using [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]]. | ||
* [[SUIT- | * [[SUIT-003_O2_ce_D050]] for living cells using [[MitoKit-CII/Succinate-nv]]. | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 10:33, 2 July 2019
- high-resolution terminology - matching measurements at high-resolution
MitoKit-CII MitoPedia
Description
Cell permeable prodrugs, composed of MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv, stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.
Abbreviation: MitoKit-CII
Reference: MitoKit-CII, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Specific SUIT protocols
- SUIT-003_O2_ce_D060 for living cells using MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv.
- SUIT-003_O2_ce_D050 for living cells using MitoKit-CII/Succinate-nv.
Application in HRR
Preparation
- Both compounds are dissolved in DMSO and stored in aliquots at -20 °C.
- We recommend the following dilutions for reaching the desired concentration in the experiments:
- MitoKit-CII/Succinate-nv: 100 mM stock solution
- MitoKit-CII/Malonate-nv: 10 mM stock solution
SUITbrowser question: Permeable succinate/malonate
- The cell permeable prodrugs Succinate-nv and Malonate-nv can help to answer questions related to the S-pathway in living cells. Use the SUITbrowser to find the best protocol to answer this and other research questions.
