MitoOx1: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=MitoOx1 | |abbr=MitoOx1 | ||
|description='''MitochondrialΒ respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials. Β | |description='''MitochondrialΒ respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials. | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
Line 12: | Line 12: | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''MitoOx1: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' Β | '''MitoOx1: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' Β | ||
::Oxygen solubility factor of | ::Oxygen solubility factor of MitoOx1 at 30Β°C and 37Β°C = 0.95 | ||
Revision as of 13:06, 23 July 2012
Description
Mitochondrial respiration medium, MitoOx1 used by the Budapest groups for respirometry und Amplex Red trials.
Abbreviation: MitoOx1
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
MitoOx1: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx1 at 30Β°C and 37Β°C = 0.95
Chemicals for MitoOx2
Compound | Final conc. | FW | Company, product code and storage |
---|---|---|---|
KCl | 125 mM | 74.55 | Sigma 31248, store at RT |
HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
KH2PO4 | 2 mM | 136.09 | Sigma P-5655, store at RT |
MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 Β°C |
adjust pH to 7.0 (room temperature) with KOH