Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MitoPedia: Oroboros QM"

From Bioblast
Line 17: Line 17:
# [[Experiment]]
# [[Experiment]]
## [[Assay]], Experimental assay
## [[Assay]], Experimental assay
## [[Assay series]], Experimental assay series
## [[Replica]]
## [[Repetitions]]
# [[Population]]
# [[Population]]
## [[Sample]]
## [[Sample]]
## [[Subsample]]
## [[Sample type]]
## [[Sample type]]
## [[Sample group]]
## [[Sample group]]

Revision as of 16:57, 11 February 2016


high-resolution terminology - matching measurements at high-resolution


MitoPedia: Oroboros QM

The MitoPedia terminology is developed continuously in the spirit of Gentle Science.


MitoFit

Template:MitoFit-QCS


Aims

The MitoFit Quality Control System (MitoFit QCS) is initiated to develop and implement standards in respirometric OXPHOS analysis, applied in comparative mitochondrial physiology, mitochondrial medicine, and clinical diagnosis.


Definitions

For a high-quality data repository, a QCS is required, based on a consistent and accessible terminology. Terms are defined in the MitoPedia system; these definitions will be formally approved by the MitoFit accreditation panel (to be formed), and will be labelled as MitoFit accredited for consistent use in the MitoFit data repository (under construction).

Examples of terms that require definition and approval are listed below:

  1. Experiment
    1. Assay, Experimental assay
    2. Replica
    3. Repetitions
  2. Population
    1. Sample
    2. Subsample
    3. Sample type
    4. Sample group
    5. Sample size
    6. Dataset
  3. MitoFit protocols
    1. Harmonization protocols (Begley, Ioannidis 2015)
    2. Normalization protocols
    3. Subprotocols
    4. Laboratory protocols
    5. Solution protocols: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc.
    6. Instrumental performance protocols
    7. Mitochondrial preparation protocols including biopsy sampling, tissue storage, cell preparation protocols, etc.
    8. Respirometric OXPHOS data acquisition and analysis protocols
  4. MitoFit registered project
    1. MitoFit pre-registered project
    2. Publicly deposited protocols (Begley, Ioannidis 2015)
    3. Prespecified protocols (Begley, Ioannidis 2015)
    4. Time-stamped protocols (Begley, Ioannidis 2015)
  5. Standardization
    1. Standard operating procedures
    2. Harmonization
  6. MitoFit proficiency test
    1. SUIT reference protocol


MitoPedia list of terms: MitoFit Quality Control System