Difference between revisions of "MitoPedia: Oroboros QM"
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== Aims == | == Aims == | ||
The MitoFit Quality Control System (MitoFit QCS) is initiated to develop and implement standards in respirometric OXPHOS analysis, applied in comparative mitochondrial physiology, mitochondrial medicine, and clinical diagnosis. | :::: The MitoFit Quality Control System (MitoFit QCS) is initiated to develop and implement standards in respirometric OXPHOS analysis, applied in comparative mitochondrial physiology, mitochondrial medicine, and clinical diagnosis. | ||
== Definitions == | == Definitions == | ||
For a high-quality data repository, a QCS is required, based on a consistent and accessible terminology. Terms are defined in the MitoPedia system; these definitions will be formally approved by the [[MitoFit accreditation panel]] (to be formed), and will be labelled as [[MitoFit accredited]] for consistent use in the [[MitoFit data repository]] (under construction). | :::: For a high-quality data repository, a QCS is required, based on a consistent and accessible terminology. Terms are defined in the MitoPedia system; these definitions will be formally approved by the [[MitoFit accreditation panel]] (to be formed), and will be labelled as [[MitoFit accredited]] for consistent use in the [[MitoFit data repository]] (under construction). | ||
Examples of terms that require definition and approval are listed below: | :::: Examples of terms that require definition and approval are listed below: | ||
# [[Experiment]] | ::::# [[Experiment]] | ||
## [[Assay]], Experimental assay | ::::## [[Assay]], Experimental assay | ||
## [[Replica]] | ::::## [[Replica]] | ||
## [[Repetitions]] | ::::## [[Repetitions]] | ||
# [[Population]] | ::::# [[Population]] | ||
## [[Sample]] | ::::## [[Sample]] | ||
## [[Subsample]] | ::::## [[Subsample]] | ||
## [[Sample type]] | ::::## [[Sample type]] | ||
## [[Sample group]] | ::::## [[Sample group]] | ||
## [[Sample size]] | ::::## [[Sample size]] | ||
## [[Dataset]] | ::::## [[Dataset]] | ||
# [[MitoFit protocols]] | ::::# [[MitoFit protocols]] | ||
## [[Harmonization protocols]] (Begley, Ioannidis 2015) | ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015) | ||
## [[Normalization protocols]] | ::::## [[Normalization protocols]] | ||
## [[Subprotocols]] | ::::## [[Subprotocols]] | ||
## [[Laboratory protocols]] | ::::## [[Laboratory protocols]] | ||
## [[Solution protocols]]: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc. | ::::## [[Solution protocols]]: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc. | ||
## [[Instrumental performance protocols]] | ::::## [[Instrumental performance protocols]] | ||
## [[Mitochondrial preparation protocols]] including biopsy sampling, tissue storage, cell preparation protocols, etc. | ::::## [[Mitochondrial preparation protocols]] including biopsy sampling, tissue storage, cell preparation protocols, etc. | ||
## [[Respirometric OXPHOS data acquisition and analysis protocols]] | ::::## [[Respirometric OXPHOS data acquisition and analysis protocols]] | ||
# [[MitoFit registered project]] | ::::# [[MitoFit registered project]] | ||
## [[MitoFit pre-registered project]] | ::::## [[MitoFit pre-registered project]] | ||
## [[Publicly deposited protocols]] (Begley, Ioannidis 2015) | ::::## [[Publicly deposited protocols]] (Begley, Ioannidis 2015) | ||
## [[Prespecified protocols]] (Begley, Ioannidis 2015) | ::::## [[Prespecified protocols]] (Begley, Ioannidis 2015) | ||
## [[Time-stamped protocols]] (Begley, Ioannidis 2015) | ::::## [[Time-stamped protocols]] (Begley, Ioannidis 2015) | ||
# [[Standardization]] | ::::# [[Standardization]] | ||
## [[Standard operating procedures]] | ::::## [[Standard operating procedures]] | ||
## [[Harmonization]] | ::::## [[Harmonization]] | ||
# [[MitoFit proficiency test]] | ::::# [[MitoFit proficiency test]] | ||
## [[SUIT reference protocol]] | ::::## [[SUIT reference protocol]] | ||
Revision as of 22:25, 17 February 2016
The MitoPedia terminology is developed continuously in the spirit of Gentle Science.
Aims
- The MitoFit Quality Control System (MitoFit QCS) is initiated to develop and implement standards in respirometric OXPHOS analysis, applied in comparative mitochondrial physiology, mitochondrial medicine, and clinical diagnosis.
Definitions
- For a high-quality data repository, a QCS is required, based on a consistent and accessible terminology. Terms are defined in the MitoPedia system; these definitions will be formally approved by the MitoFit accreditation panel (to be formed), and will be labelled as MitoFit accredited for consistent use in the MitoFit data repository (under construction).
- Examples of terms that require definition and approval are listed below:
- Experiment
- Assay, Experimental assay
- Replica
- Repetitions
- Population
- MitoFit protocols
- Harmonization protocols (Begley, Ioannidis 2015)
- Normalization protocols
- Subprotocols
- Laboratory protocols
- Solution protocols: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc.
- Instrumental performance protocols
- Mitochondrial preparation protocols including biopsy sampling, tissue storage, cell preparation protocols, etc.
- Respirometric OXPHOS data acquisition and analysis protocols
- MitoFit registered project
- MitoFit pre-registered project
- Publicly deposited protocols (Begley, Ioannidis 2015)
- Prespecified protocols (Begley, Ioannidis 2015)
- Time-stamped protocols (Begley, Ioannidis 2015)
- Standardization
- MitoFit proficiency test
- Experiment