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Difference between revisions of "Mitochondrial membrane potential"

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{{MitoPedia
{{MitoPedia
|abbr=mtMP, ฮ”''ฯˆ''<sub>mt</sub>
|abbr=mtMP, ฮ”''ฯˆ''<sub>mt</sub>
|description=
|description=The '''mitochondrial membrane potential''', mtMP, is the electric part of the protonmotive force, ฮ”''p''<sub>mt</sub>.
The '''mitochondrial membrane potential''', mtMP, is the electric part of the protonmotive force, ฮ”''p''<sub>mt</sub>.


ฮ”''ฯˆ''<sub>mt</sub> ๏„= ฮ”''p''<sub>mt</sub> - ฮ”''ยต''<sub>H+</sub> / ''F''
ฮ”''ฯˆ''<sub>mt</sub> ๏„= ฮ”''p''<sub>mt</sub> - ฮ”''ยต''<sub>H+</sub> / ''F''
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''F'' = 96.4853 kJol/V = 96,485.3 C/mol
''F'' = 96.4853 kJol/V = 96,485.3 C/mol
|info=[[Mitchell 1961 Nature]], [[Gnaiger 2014 Preface MiP2014]]
|info=[[Mitchell 1961 Nature]], [[Gnaiger 2014 Preface MiP2014]]
}}
{{MitoPedia concepts
|mitopedia concept=Respiratory state
}}
}}
{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry, Fluorometry
|mitopedia method=Respirometry, Fluorometry
}}
}}
{{MitoPedia topics
{{MitoPedia O2k and high-resolution respirometry}}
|mitopedia topic=Respiratory state
{{MitoPedia topics}}
}}
{{Template:Technical support integrated}}
{{Template:Technical support integrated}}
=== Different methods for measurements of mt-membrane potential ===
=== Different methods for measurements of mt-membrane potential ===

Revision as of 14:01, 8 February 2016


high-resolution terminology - matching measurements at high-resolution


Mitochondrial membrane potential

Description

The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ฮ”pmt.

ฮ”ฯˆmt = ฮ”pmt - ฮ”ยตH+ / F

mtMP or ฮ”ฯˆmt is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per โ€˜motiveโ€™ electron or per electron moved across the transmembrane potential difference, with the number of โ€˜motiveโ€™ electrons expressed in the unit coulomb [C].

The chemical part of the protonmotive force, ยตH+ / F stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],

F = 96.4853 kJol/V = 96,485.3 C/mol

Abbreviation: mtMP, ฮ”ฯˆmt

Reference: Mitchell 1961 Nature, Gnaiger 2014 Preface MiP2014


MitoPedia concepts: Respiratory state 


MitoPedia methods: Respirometry, Fluorometry 





Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Different methods for measurements of mt-membrane potential

Mt-membrane potential can either be measured with the O2k-Fluo LED2-Module by using the fluorophores TMRM or Safranin or with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion reporters inhibit respiration, which makes it essential to test the optimum concentration before starting your experimenal series.

ยป MiPNet15.03 O2k-MultiSensor-ISE
ยป MiPNet17.05 O2k-Fluo LED2-Module