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Difference between revisions of "Mitochondrial membrane potential"

From Bioblast
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|info=[[Mitchell 1961 Nature]], [[Gnaiger 2014 Preface MiP2014]]
|info=[[Mitchell 1961 Nature]], [[Gnaiger 2014 Preface MiP2014]]
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Communicated by [[Gnaiger E]] 2012-10-05, edited 2016-02-06, 2017-09-05.
{{Template:Technical support integrated}}
=== Different methods for measurements of mt-membrane potential ===
:::: Mt-membrane potential can either be measured with the [[O2k-Fluo LED2-Module]] by using the fluorophores [[TMRM]] or [[Safranin]] or with the [[O2k-TPP+ ISE-Module]] electrode by using the ion reporter [[TPP+]]. All mentioned ion reporters inhibit respiration, which makes it essential to test the optimum concentration before starting your experimenal series.
::::» [[MiPNet15.03 O2k-MultiSensor-ISE]]
::::» [[MiPNet17.05 O2k-Fluo LED2-Module]]
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=Respiratory state, Recommended, Ergodynamics
|mitopedia concept=Respiratory state, Recommended, Ergodynamics
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|mitopedia method=Respirometry, Fluorometry
|mitopedia method=Respirometry, Fluorometry
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{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=EAGLE
|mitopedia topic=EAGLE
}}
}}
Communicated by [[Gnaiger E]] 2012-10-05, edited 2016-02-06, 2017-09-05.
{{Template:Technical support integrated}}
=== Different methods for measurements of mt-membrane potential ===
:::: Mt-membrane potential can either be measured with the [[O2k-Fluo LED2-Module]] by using the fluorophores [[TMRM]] or [[Safranin]] or with the [[O2k-TPP+ ISE-Module]] electrode by using the ion reporter [[TPP+]]. All mentioned ion reporters inhibit respiration, which makes it essential to test the optimum concentration before starting your experimenal series.
::::» [[MiPNet15.03 O2k-MultiSensor-ISE]]
::::» [[MiPNet17.05 O2k-Fluo LED2-Module]]

Revision as of 20:17, 23 August 2018


high-resolution terminology - matching measurements at high-resolution


Mitochondrial membrane potential

Description

The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.

Δψ = ΔpH+ - ΔµH+ / F

mtMP or Δψ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ electron or per electron moved across the transmembrane potential difference, with the number of ‘motive’ electrons expressed in the unit coulomb [C].

The chemical part of the protonmotive force, µH+ / F stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],

F = 96.4853 kJol/V = 96,485.3 C/mol

Abbreviation: mtMP, Δψ

Reference: Mitchell 1961 Nature, Gnaiger 2014 Preface MiP2014

Communicated by Gnaiger E 2012-10-05, edited 2016-02-06, 2017-09-05.


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Different methods for measurements of mt-membrane potential

Mt-membrane potential can either be measured with the O2k-Fluo LED2-Module by using the fluorophores TMRM or Safranin or with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion reporters inhibit respiration, which makes it essential to test the optimum concentration before starting your experimenal series.
» MiPNet15.03 O2k-MultiSensor-ISE
» MiPNet17.05 O2k-Fluo LED2-Module


MitoPedia concepts: Respiratory state, Recommended, Ergodynamics 


MitoPedia methods: Respirometry, Fluorometry 


MitoPedia topics: EAGLE