Difference between revisions of "Moura AS FASEB 2011"
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{{Abstract | {{Abstract | ||
|title=Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V | |title=Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V, Sichieri R (2011) Mitochondrial dysfunction in cardiomyocytes of obese mice. FASEB J 25: 1028.3 | ||
|info=[http://www.fasebj.org/cgi/content/meeting_abstract/25/1_MeetingAbstracts/1028.3?sid=103d1a34-411b-43a3-9a86-4996fbcde031 PDF] | |info=[http://www.fasebj.org/cgi/content/meeting_abstract/25/1_MeetingAbstracts/1028.3?sid=103d1a34-411b-43a3-9a86-4996fbcde031 PDF] | ||
|authors=Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V, Sichieri R | |authors=Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V, Sichieri R | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
| | |area=Respiration | ||
|organism=Mouse | |organism=Mouse | ||
|tissues=Heart | |tissues=Heart | ||
|preparations=Intact | |preparations=Intact cells | ||
|instruments=Oxygraph-2k | |||
}} | }} | ||
Latest revision as of 15:43, 4 June 2017
| Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V, Sichieri R (2011) Mitochondrial dysfunction in cardiomyocytes of obese mice. FASEB J 25: 1028.3 |
Link: PDF
Moura AS, Cortez E, Bernardo A, Garcia-Souza E, Neves F, Mattos A, Miranda G, Soares V, Sichieri R (2011)
Event: FASEB J
Cellular energy metabolism is impaired in obesity. Therefore in this study it was investigated cardiomyocyte bioenergetics and ultrastructure of adult obese mice overfed during lactation.
We performed high-resolution respirometry of cardiomyocytes from obese (OG) and control (CG) groups with an Oroboros 2k-Oxygraph. State 2 (absence of ADP), state 3 (ADP-stimulated) and state 4 respiration were examined with NAD-linked substrates or fatty acid. Cardiomyocytes ultrastructure was analyzed by transmission electron microscopy. The content of mitochondrial proteins Carnitine palmitoil transferase 1 (CPT1) and Uncoupling protein 2 (UCP2) was achieved by western blotting. Statistical significance was assessed by analysis of variance (ANOVA) followed by Tukey’s test; P<0.05.
OG presented higher body weight and epididymal fat than CG (P<0.0001). O2 consumption was not different between groups using NAD-linked substrates but significantly lower in OG when palmitoil-carnitine was added as substrate (P<0.0001). CPT1 and UCP2 content was found significantly increased in OG (P<0.05). Ultrastructure analysis showed disrupted mitochondria in OG and also a higher ectopic lipid deposition then CG.
We suggest that early life overnutrition leads to obesity associated with cardiomyocytes bioenergetics and ultrastructure impairment. Supported by CNPq (305903/2007); FAPERJ(E-26/110.578/2009).
• Keywords: obesity; cardiomyocytes;
• O2k-Network Lab: BR Rio de Janeiro Moura AS
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Intact cells
HRR: Oxygraph-2k
