Mufazalov 2009 Mol Biol (Mosk)
| Mufazalov IA, Pen'kov DN, Cherniak BV, Pletiushkina OIu, Vysokikh MIu, Kirpichnikov MP, Dolgikh DA, Kruglov AA, Kuprash DV, Skulachev VP, Nedospasov SA (2009) Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene. Mol Biol (Mosk) 43: 648-656. |
Mufazalov IA, Pen'kov DN, Cherniak BV, Pletiushkina OIu, Vysokikh MIu, Kirpichnikov MP, Dolgikh DA, Kruglov AA, Kuprash DV, Skulachev VP, Nedospasov SA (2009) Mol Biol (Mosk)
Abstract: Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome C gene expression or its localization inside the cell. These cell cultures therefore represent an interesting model for study apoptotic signaling and physiological functions of cytochrome C. • Keywords: [Article in Russian]; staurosporin
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Stress:z in prep"z in prep" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Mouse Tissue;cell: Fibroblast Preparation: z in prep"z in prep" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. Enzyme: z in prep"z in prep" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: z in prep"z in prep" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
