Difference between revisions of "Myxothiazol"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Myx | |abbr=Myx | ||
|description='''Myxothiazol''' is an inhibitor of [[Complex III | |description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII inhibits also [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} | ||
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'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol): | '''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol): | ||
: | :# Weigh 1 mg of myxothiazol into a small glass vial. | ||
: | :# Add 2.05 ml ethanol. | ||
: | :# Divide into 0.2 ml (use glass vials). | ||
: | :# Store at -20 °C. | ||
'''Oxygraph-2k manual titrations''' [[MiPNet09.12]] | '''Oxygraph-2k manual titrations''' [[MiPNet09.12]] | ||
:* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | |||
:* Final concentration: 0.5 µM. | |||
== Compare == | == Compare == | ||
* [[Antimycin A]] | * [[Antimycin A]] is less expensive than Myx but induces higher ROS production. |
Revision as of 09:18, 12 October 2015
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII inhibits also CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 ml ethanol.
- Divide into 0.2 ml (use glass vials).
- Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.