Difference between revisions of "O2k-Titration Set"
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|description=[[Image:O2k-Titration Set.JPG|right|180px]] The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 1 x 50 mm<sup>3</sup>, 1 x 100 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>; fixed needles with rounded tips), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]]. | |description=[[Image:O2k-Titration Set.JPG|right|180px]] The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 1 x 50 mm<sup>3</sup>, 1 x 100 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>; fixed needles with rounded tips), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]]. | ||
|info= [https://www.oroboros.at/index.php/product/o2k-titration-set/ '''Product details and purchasing information'''] | |info= [https://www.oroboros.at/index.php/product/o2k-titration-set/ '''Product details and purchasing information'''] | ||
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Revision as of 10:25, 25 August 2022
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O2k-Titration Set |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks.
Reference: Product details and purchasing information
Additional resources
Note: Titration of chemicals in respirometry
- Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
Further information
- Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
- Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
- Visit also: Hamilton.
- Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
- Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gently down.
MitoPedia O2k and high-resolution respirometry: O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
