Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "O2k-Titration Set"

From Bioblast
 
(29 intermediate revisions by 7 users not shown)
Line 1: Line 1:
{{Product
{{Technical support}}
|description=The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 2 x 50 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]].  
__TOC__
The O2k-Titration Set is a basic HRR-Accessory included in the [[O2k-Core]].
{{MitoPedia without banner
|product id=20820-02
|abbr=
|product type=O2k, O2k-Module, O2k-Core, Microsyringe, Catalogue
|description=[[Image:O2k-Titration Set.JPG|right|180px]] The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 1 x 50 mm<sup>3</sup>, 1 x 100 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>; fixed needles with rounded tips), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]].  
|info=[[MiPNet09.12 O2k-Titrations]], [[MiPNet19.14 SOP Hamilton microsyringes]]
 
|product image=[[Image:O2k-Titration Set.JPG|right|180px]]
|info= [https://www.oroboros.at/index.php/product/o2k-titration-set/ '''Product details and purchasing information''']
}}
== The '''O2k-Titration Set''' ''consists of''==
{{#ask: [[Category:Products]] [[Product type::Titration Set]]
| ?description
| ?product id
| ?product image
|sort=product id
|order=ascending
|mainlabel=Title
}}
}}
== Additional resources ==
:::: »[[MiPNet09.12 O2k-Titrations]] </br>
:::: »[[MiPNet19.14 SOP Hamilton microsyringes]]




[[File:Tube and Syringe Racks with O2k.JPG|left|240px|Tube and Syringe Racks with O2k]]
== Note: Titration of chemicals in respirometry ==
: 6 [[Microsyringe\10 mm3 51/0.13 mm]] 10 mm<sup>3</sup>
:::: Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
: 6 [[Microsyringe\25 mm3 51/0.15 mm]] 25 mm<sup>3</sup>
: 2 [[Microsyringe\50 mm3 51/0.15 mm]] 50 mm<sup>3</sup>
: 1 [[Syringe\500 mm3 51/0.41 mm]] 500 mm<sup>3</sup> - '''''new''''', for injection of isolated mitochondria or suspended cells; for filling the new 200 mm<sup>3</sup> [[Microsyringe\200 mm3\TIP2k |TIP2k microsyringes]].
: 3 [[Plunger\10 mm3]] for 10 mm<sup>3</sup> syringe
: 1 package of two [[Syringe Racks]] including 20 [[Syringe Collars]] - '''''new'''''
: 1 package of two [[Tube Racks]] including 8 tubes 50 ml - '''''new'''''
: 1 [[Syringe Storage Box]] including [[Syringe Labels]] - '''''new'''''


::::  After a titration, it is common to observe liquid accumulated on top of the stopper receptacle. When this happens, we recommend removing this excess of liquid as it can lead to O<sub>2</sub> backdiffusion into the chamber. For this, we recommend using the [[ISS]], placing the tip close to the capillary, but not directly on top of it.


::» Go to [[OROBOROS O2k-Catalogue]], [http://www.oroboros.at/?Purchase Order @OROBOROS]
=== Further information ===
:::* Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
:::* Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
:::* Visit also: [http://www.hamiltoncompany.com/products/syringes-and-needles Hamilton].
:::* Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
:::* Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H<sub>2</sub>O and try to press the plunger gently down.




=== Further information ===
{{MitoPedia O2k and high-resolution respirometry
:::* [[MiPNet19.14 SOP Hamilton microsyringes | MiPNet19.14]] - SOP for manual O2k-titrations with Hamilton microsyringes
|mitopedia O2k and high-resolution respirometry=O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
:::* Hamilton microsyringes are specifically produced for O2k-titrations  and can be ordered directly from OROBOROS INSTRUMENTS to assure exact  fitting with the O2k-Stopper and O2k-Chamber dimensions.
}}
:::* Visit also: [http://www.hamiltoncompany.com/products/syringes-and-needles Hamilton] 
:::* Eliminate rugh edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing
:::* Use a cleaning wire for the needle. If a syringe is clocked , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gentle down.

Latest revision as of 16:46, 11 October 2022


                  


O2k-Open Support

O2k-Titration Set



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

O2k-Titration Set.JPG

The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks.


Reference: Product details and purchasing information


Additional resources

»MiPNet09.12 O2k-Titrations
»MiPNet19.14 SOP Hamilton microsyringes


Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
After a titration, it is common to observe liquid accumulated on top of the stopper receptacle. When this happens, we recommend removing this excess of liquid as it can lead to O2 backdiffusion into the chamber. For this, we recommend using the ISS, placing the tip close to the capillary, but not directly on top of it.

Further information

  • Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
  • Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
  • Visit also: Hamilton.
  • Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
  • Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gently down.


MitoPedia O2k and high-resolution respirometry: O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry