|
|
| (24 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| {{Mainpage OROBOROS}}
| | #REDIRECT [[O2k-Workshops]] |
| | |
| __TOC__
| |
| | |
| == General contents of O2k-Workshops: Overview on High-Resolution Respirometry ==
| |
| | |
| Introduction: Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
| |
| | |
| High-resolution respirometry; what makes the difference? Presentation of the OROBOROS Oxygraph-2k
| |
| | |
| | |
| * [http://www.oroboros.at/index.php?id=oxygraph-2k| Optimum system design] - the OROBOROS Oxygraph-2k.
| |
| * [http://www.oroboros.at/index.php?id=datlab| DatLab]: on-line recording of oxygen concentration and flux; linear slope versus oxygen flux as a function of time.
| |
| * DatLab: the specialized software for high-resolution respirometry; high resolution calibrations.
| |
| * Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.
| |
| | |
| | |
| '''O2k and TIP2k: On-line instrumental performance'''
| |
| | |
| * [http://www.oroboros.at/index.php?id=backgroundcorrection| Instrumental background]: measurement and correction as a function of pO2.
| |
| * High-resolution of [http://www.oroboros.at/index.php?id=uncouplertitrations|respiratory flux at various steady-states].
| |
| * O2k-MultiSensor applications: TPP+, pH, NO
| |
| * [http://www.oroboros.at/index.php?id=tip2k| The Titration-Injection microPump TIP2k]: automatic titrations.
| |
| * Conceptual and methodological advantages of measurement at physiological low levels of oxygen.
| |
| | |
| | |
| '''[[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service'''
| |
| | |
| * Cleaning of anode and cathode.
| |
| * Electrolyte and membrane application.
| |
| * O2k and TIP2k: instrumental maintenance.
| |
| | |
| | |
| ==Formats==
| |
| | |
| === O2k-Workshop: HRR and O2k-Fluorometry ===
| |
| | |
| The O2k-Workshop on HRR and Fluorometry provides a detailed introduction to the OROBOROS Oxygraph-2k ([http://www.oroboros.at/index.php?id=oxygraph-2k| O2k-Core]), overview presentations on high-resolution respirometry ([http://www.oroboros.at/index.php?id=oxygraph| HRR]), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.
| |
| | |
| The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; [http://www.oroboros.at/index.php?id=mipnet-protocols| substrate-uncoupler-inhibitor titration SUIT protocols]). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects
| |
| | |
| | |
| | |
| === O2k-Workshop: O2k-Fluorometry ===
| |
| | |
| [[File:IOC75 19.JPG|180px|right]]
| |
| | |
| The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production [[Amplex red| (Amplex red)]], mt-membrane potential [[Safranin|(Safranin)]], ATP production [[Magnesium green| (Mg green)]], and Ca2+ [[Calcium green| (Ca green)]].
| |
| | |
| Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.
| |
| | |
| Information on O2k-Fluorometry, with resolution of hydrogenperoxide in the nanomolar range: [[O2k-Fluo LED2-Module| O2k-Fluorescence LED2-Module]].
| |
| | |
| Moreover, O2k-Workshops with a special focus on O2k-Fluorometry are offered (e.g. [[IOC95| IOC95)]]. Further O2k-MultiSensor applications are covered in these specific O2k-Workshops
| |
| | |
| * Measurement of [[Calcium electrode|Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
| |
| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
| |
| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
| |
| | |
| | |
| === O2k-Workshop: TPP/ ISE ===
| |
| [[Image:O2k-TPP-ISE.jpg|180px|right]]
| |
| The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of [http://www.oroboros.at/index.php?membrane-potential| mitochondrial membrane potential] (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
| |
| | |
| According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:
| |
| | |
| * [[Calcium electrode| Measurement of Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
| |
| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
| |
| * [http://www.oroboros.at/index.php?id=o2k-fluorescence| O2k-Fluorescence]
| |
| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
| |
