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| {{Mainpage OROBOROS}}
| | #REDIRECT [[O2k-Workshops]] |
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| __TOC__
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| == General contents of O2k-Workshops ==
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| * Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance
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| * High-resolution respirometry
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| * [http://www.oroboros.at/index.php?id=oxygraph-2k| Optimum system design] - the OROBOROS Oxygraph-2k.
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| * [http://www.oroboros.at/index.php?id=datlab| DatLab]: on-line recording of oxygen concentration and flux; linear slope versus oxygen flux as a function of time.
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| * DatLab: the specialized software for high-resolution respirometry; high resolution calibrations.
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| * Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.
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| '''O2k and TIP2k: On-line instrumental performance'''
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| * [http://www.oroboros.at/index.php?id=backgroundcorrection| Instrumental background]: measurement and correction as a function of pO2.
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| * High-resolution of [http://www.oroboros.at/index.php?id=uncouplertitrations|respiratory flux at various steady-states].
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| * O2k-MultiSensor applications: TPP+, pH, NO
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| * [http://www.oroboros.at/index.php?id=tip2k| The Titration-Injection microPump TIP2k]: automatic titrations.
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| * Conceptual and methodological advantages of measurement at physiological low levels of oxygen.
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| '''[[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service'''
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| * Cleaning of anode and cathode.
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| * Electrolyte and membrane application.
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| * O2k and TIP2k: instrumental maintenance.
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| == [[OROBOROS_O2k-Workshops#Next_O2k-Workshops| List of O2k Workshops]] ==
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| ==Formats==
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| === O2k-Workshop: HRR and O2k-Fluorometry ===
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| The O2k-Workshop on HRR and Fluorometry provides a detailed introduction to the OROBOROS Oxygraph-2k ([http://www.oroboros.at/index.php?id=oxygraph-2k| O2k-Core]), overview presentations on high-resolution respirometry ([http://www.oroboros.at/index.php?id=oxygraph| HRR]), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.
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| The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; [http://www.oroboros.at/index.php?id=mipnet-protocols| substrate-uncoupler-inhibitor titration SUIT protocols]). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects
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| === O2k-Workshop: O2k-Fluorometry ===
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| [[File:IOC75 19.JPG|180px|right]]
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| The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production [[Amplex red| (Amplex red)]], mt-membrane potential [[Safranin|(Safranin)]], ATP production [[Magnesium green| (Mg green)]], and Ca2+ [[Calcium green| (Ca green)]].
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| Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.
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| Information on O2k-Fluorometry, with resolution of hydrogenperoxide in the nanomolar range: [[O2k-Fluo LED2-Module| O2k-Fluorescence LED2-Module]].
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| Moreover, O2k-Workshops with a special focus on O2k-Fluorometry are offered (e.g. [[IOC95| IOC95)]]. Further O2k-MultiSensor applications are covered in these specific O2k-Workshops
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| * Measurement of [[Calcium electrode|Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
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| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
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| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
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| === O2k-Workshop: TPP/ ISE ===
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| [[Image:O2k-TPP-ISE.jpg|180px|right]]
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| The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of [http://www.oroboros.at/index.php?membrane-potential| mitochondrial membrane potential] (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
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| According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:
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| * [[Calcium electrode| Measurement of Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
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| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
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| * [http://www.oroboros.at/index.php?id=o2k-fluorescence| O2k-Fluorescence]
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| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
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| === [[O2k-Workshops in MiPNet Labs]] ===
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| == OROBOROS Travel Grant ==
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| OROBOROS INSTRUMENTS offers travel grants for the support of scientists attending the O2k-Workshop on High-Resolution Respirometry (HRR).
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| '''Eligibility and Application'''
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| * With the purchase of an O2k, you are eligible for receiving an OROBOROS Travel Grant.
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| * Specific academic OROBOROS Travel Grants are availble as start-up support. Scientists establishing a new lab in their first university position may apply by sending a letter of confirmation by their university.
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| '''General Information'''
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| * The number of OROBOROS Travel Grants is limited.
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| * OROBOROS Travel Grants are limited to one per research group.
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| * The selection of awardees is made by OROBOROS INSTRUMENTS.
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| == [[IOC Recommended Reading]] ==
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| == Contact ==
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| OROBOROS INSTRUMENTS
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| high-resolution respirometry
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| Schöpfstrasse 18
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| A-6020 Innsbruck, Austria
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| Tel: +43 512 566796
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| Fax: +43 512 566796 20
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