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| {{Mainpage OROBOROS}}
| | #REDIRECT [[O2k-Workshops]] |
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| __TOC__
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| == General contents of O2k-Workshops ==
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| * Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance
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| * High-resolution respirometry
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| * [http://www.oroboros.at/index.php?id=oxygraph-2k| Optimum system design] - the OROBOROS Oxygraph-2k.
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| * [http://www.oroboros.at/index.php?id=datlab| DatLab]: on-line recording of oxygen concentration and flux; linear slope versus oxygen flux as a function of time.
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| * DatLab: the specialized software for high-resolution respirometry; high resolution calibrations.
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| * Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.
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| '''O2k and TIP2k: On-line instrumental performance'''
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| * [http://www.oroboros.at/index.php?id=backgroundcorrection| Instrumental background]: measurement and correction as a function of pO2.
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| * High-resolution of [http://www.oroboros.at/index.php?id=uncouplertitrations|respiratory flux at various steady-states].
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| * O2k-MultiSensor applications: TPP+, pH, NO
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| * [http://www.oroboros.at/index.php?id=tip2k| The Titration-Injection microPump TIP2k]: automatic titrations.
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| * Conceptual and methodological advantages of measurement at physiological low levels of oxygen.
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| '''[[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service'''
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| * Cleaning of anode and cathode.
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| * Electrolyte and membrane application.
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| * O2k and TIP2k: instrumental maintenance.
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| == [[OROBOROS_O2k-Workshops#Next_O2k-Workshops| List of O2k Workshops]] ==
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| ==Formats==
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| === O2k-Workshop: HRR and O2k-Fluorometry ===
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| The O2k-Workshop on HRR and Fluorometry provides a detailed introduction to the OROBOROS Oxygraph-2k ([http://www.oroboros.at/index.php?id=oxygraph-2k| O2k-Core]), overview presentations on high-resolution respirometry ([http://www.oroboros.at/index.php?id=oxygraph| HRR]), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.
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| The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; [http://www.oroboros.at/index.php?id=mipnet-protocols| substrate-uncoupler-inhibitor titration SUIT protocols]). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects
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| === O2k-Workshop: O2k-Fluorometry ===
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| [[File:IOC75 19.JPG|180px|right]]
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| The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production [[Amplex red| (Amplex red)]], mt-membrane potential [[Safranin|(Safranin)]], ATP production [[Magnesium green| (Mg green)]], and Ca2+ [[Calcium green| (Ca green)]].
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| Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.
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| Information on O2k-Fluorometry, with resolution of hydrogenperoxide in the nanomolar range: [[O2k-Fluo LED2-Module| O2k-Fluorescence LED2-Module]].
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| Moreover, O2k-Workshops with a special focus on O2k-Fluorometry are offered (e.g. [[IOC95| IOC95)]]. Further O2k-MultiSensor applications are covered in these specific O2k-Workshops
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| * Measurement of [[Calcium electrode|Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
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| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
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| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
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| === O2k-Workshop: TPP/ ISE ===
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| [[Image:O2k-TPP-ISE.jpg|180px|right]]
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| The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of [http://www.oroboros.at/index.php?membrane-potential| mitochondrial membrane potential] (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
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| According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:
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| * [[Calcium electrode| Measurement of Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
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| * [[O2k-pH ISE-Module|Acidification rate (pH)]]
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| * [http://www.oroboros.at/index.php?id=o2k-fluorescence| O2k-Fluorescence]
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| * [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
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| === [[O2k-Workshops in MiPNet Labs]] ===
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| == Abstracts ==
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| Abstracts are welcome for short presentations, providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
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| '''Instructions for IOC-Abstracts'''
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| Abstracts are welcome for short presentations (hot topics in mitochondrial physiology), providing participants an opportunity to present and discuss their work, including projects in progress or in the planning state, methodological details and conceptual aspects.
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| * '''Length of text''': Max. 500 words including up to three references (all authors, full title) and a figure legend. '''''Attention''''': The website does not allow excess length of text. Additional information (further references etc.) may be added into the free text which will not be included in the printed version.
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| * You may edit your abstract on the website any time. However, please consider the '''deadline for the printed programme''':
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| :* '''O2k-Fluorometry Workshop Sep 2014, Obergurgl - deadline: 2014-08-11'''
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| :* '''O2k-Workshop on HRR and O2k-Fluorometry Oct 2014, Schroecken - deadline: 2014-09-01'''
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| * Kindly submit your abstract by emailing it to us in a wordfile. We will then add your abstract to the bioblast wiki. Please let us know if you do not wish your abstract to be open access.
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| * '''Figures''': A figure is welcome. Please send us a '''figure''' (jpg or png) and your '''photo''' (jpg or png).
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| == OROBOROS Travel Grant ==
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| OROBOROS INSTRUMENTS offers travel grants for the support of scientists attending the O2k-Workshop on High-Resolution Respirometry (HRR).
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| '''Eligibility and Application'''
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| * With the purchase of an O2k, you are eligible for receiving an OROBOROS Travel Grant.
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| * Specific academic OROBOROS Travel Grants are availble as start-up support. Scientists establishing a new lab in their first university position may apply by sending a letter of confirmation by their university.
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| '''General Information'''
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| * The number of OROBOROS Travel Grants is limited.
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| * OROBOROS Travel Grants are limited to one per research group.
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| * The selection of awardees is made by OROBOROS INSTRUMENTS.
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| == [[IOC Recommended Reading| Recommended Reading]] ==
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| == Contact ==
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| OROBOROS INSTRUMENTS
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| high-resolution respirometry
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| Schöpfstrasse 18
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| A-6020 Innsbruck, Austria
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| Tel: +43 512 566796
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| Fax: +43 512 566796 20
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