Difference between revisions of "Octanoylcarnitine"

Revision as of 09:58, 12 September 2019

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Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite

» Fatty acid oxidation

» Carnitine O-octanoyltransferase

Application in HRR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H₂O.
Divide into 0.2 mL portions.
Store at -20 °C.

O2k manual titrations into 2 mL O2k-chamber

Titration volume: 10 µL using a 10 µL syringe.
Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.

@@ Line 28: / Line 28: @@
 ::: '''O2k manual titrations into 2 mL O2k-chamber'''
-::::* Titration volume: 4 µL using a 10 µL syringe.
+::::* Titration volume: 10 µL using a 10 µL syringe.
-::::* Final concentration: 0.2 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
+::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.