Difference between revisions of "Palenickova 2011 Physiol Res"
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|abstract=Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary. | |abstract=Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary. | ||
|keywords=liver homogenate, glutamate and palmityl carnitine oxidation, metformin | |keywords=liver homogenate, glutamate and palmityl carnitine oxidation, metformin | ||
|mipnetlab=CZ Prague Houstek J, CZ Prague Bioenergetics | |mipnetlab=CZ Prague Houstek J, CZ Prague Bioenergetics, CZ Prague Kalous M | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 12:28, 8 January 2013
| Palenickova E, Cahova M, Drahota Z, Kazdova L, Kalous M (2011) Inhibitory effect of metformin on oxidation of NADH-dependent substrates in rat liver homogenate. Physiol Res 60: 835-839. |
Palenickova E, Cahova M, Drahota Z, Kazdova L, Kalous M (2011) Physiol Res
Abstract: Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary. • Keywords: liver homogenate, glutamate and palmityl carnitine oxidation, metformin
• O2k-Network Lab: CZ Prague Houstek J, CZ Prague Bioenergetics, CZ Prague Kalous M
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
