Difference between revisions of "Palmeira 2012 Methods Mol Biol"
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Revision as of 12:40, 8 February 2018
| Palmeira CM, Rolo AP (2012) Mitochondrial membrane potential (ΔΨ) fluctuations associated with the metabolic states of mitochondria. Methods Mol Biol 810:89-101. |
Palmeira CM, Rolo AP (2012) Methods Mol Biol
Abstract: The study of the mitochondrial membrane potential (ΔΨ) is essential for an integrated appraisal of mitochondrial function, since it reflects differences in electrical potential and represents the main component of the proton electrochemical gradient accounting for more than 90% of the total available respiratory energy. Numerous methods have been used to estimate mitochondrial membrane potential (ΔΨ), including fluorescent methods and electrochemical probes. In this chapter, we describe several practical approaches that allow mitochondrial membrane potential (ΔΨ) evaluation, by using a tetraphenylphosphonium (TPP(+))-selective electrode. The main focus is given to the evaluation of ΔΨ in isolated mitochondria. • Keywords: TPP+-selective electrode, membrane potential, mitochondria, permeabilized cells, metabolic states, permeability transition • Bioblast editor: Sumbalova Z • O2k-Network Lab: PT Coimbra Laranjinha J
Labels: MiParea: Instruments;methods
Stress:Permeability transition, Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: ATP production, Calcium, mt-Membrane potential, Substrate, Uncoupler, Q-junction effect
HRR: TPP, Theory
