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Difference between revisions of "Palmitoylcarnitine"

From Bioblast
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== Application in [[HRR]] ==
== Application in [[HRR]] ==


'''Pal: Palmitoylcarnitine'''  (Palmitoyl-DL-carnitine-HCl, C<sub>23</sub>H<sub>45</sub>N0<sub>4</sub>HCl); Sigma P 4509, 100 mg, store at -20 °C; FW = 436.1.
:::'''Pal: Palmitoylcarnitine'''  (Palmitoyl-DL-carnitine-HCl, C<sub>23</sub>H<sub>45</sub>N0<sub>4</sub>HCl); Sigma P 4509, 100 mg, store at -20 °C; FW = 436.1.


'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):  
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):  
 
::::# Weigh 8.72 mg of palmitoylcarnitine.
::::# Dissolve in 2 mL H<sub>2</sub>O.
::::# Divide into 0.2 mL portions.
::::# Store frozen at -20 °C.
 
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
 
::::* Titration volume: 8 µL using a 25 µL syringe (2 mL O2k-chamber).
::::* Final concentration: 0.04 mM.


::1) Weigh 8.72 mg of palmitoylcarnitine.
::2) Dissolve in 2 mL H<sub>2</sub>O.
::3) Divide into 0.2 mL portions.
::4) Store frozen at -20 °C.
{{MitoPedia methods|type=Substrate ETS
{{MitoPedia methods|type=Substrate ETS
}}
}}

Revision as of 14:12, 17 September 2020


high-resolution terminology - matching measurements at high-resolution


Palmitoylcarnitine

Description

Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.

Abbreviation: Pal

Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.


Application in HRR

Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl, C23H45N04HCl); Sigma P 4509, 100 mg, store at -20 °C; FW = 436.1.
Preparation of 10 mM stock solution (dissolved in H2O):
  1. Weigh 8.72 mg of palmitoylcarnitine.
  2. Dissolve in 2 mL H2O.
  3. Divide into 0.2 mL portions.
  4. Store frozen at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 8 µL using a 25 µL syringe (2 mL O2k-chamber).
  • Final concentration: 0.04 mM.



MitoPedia topics: Substrate and metabolite